Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606132039_gtfg CCATGCGGCTCAGCCATGCTGGTCTCTCCGAG(+AAAAAA)GTACCCAGATAGCTGCACCCCTCTGCTCCCC NGG 10 8 17 8 24 0 100 5 10
    240606132037_b70q AGCAAGGGGAGCAGAGGGGTGCAGCTATCTGG(+AAAAAA)GTACCTCGGAGAGACCAGCATGGCTGAGCCG NGG 10 8 17 8 24 0 100 5 10
    240606132035_v99j ACTGTCTTCAGACACAGCAGAAGAGGGCATCG(+AAAAAA)GATCCCATTACAGACTTGAGCCACCCCGTGG NGG 10 8 17 8 24 0 100 5 10
    240606132034_599l AGTACCCACGGGGTGGCTCAAGTCTGTAATGG(+AAAAAA)GATCCGATGCCCTCTTCTGCTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606132033_c3k9 GGCACATGGCCAACCTGTTGGGTTGGTTTTTG(+AAAAAA)GTACCTAAGGGACTTTGGAAGAAGGATCAAG NGG 10 8 17 8 24 0 100 5 10
    240606132031_hr5q GGGTTCTTGATCCTTCTTCCAAAGTCCCTTAG(+AAAAAA)GTACCAAAAACCAACCCAACAGGTTGGCCAT NGG 10 8 17 8 24 0 100 5 10
    240606132029_rqok AGAGGATACGTGGCTTGAAGCAGCTGCAGAGG(+AAAAAA)GTACCTTCAGCCTTGAGCCACACTCTTAAAT NGG 10 8 17 8 24 0 100 5 10
    240606132028_ont8 TTCTGATTTAAGAGTGTGGCTCAAGGCTGAAG(+AAAAAA)GTACCCTCTGCAGCTGCTTCAAGCCACGTAT NGG 10 8 17 8 24 0 100 5 10
    240606132026_byxq CCCCAGTAAAGAAAAGAGAGCAAAAAACACTG(+AAAAAA)GTACCCGCCTTGCTTTCTCCTTTATACAGGA NGG 10 8 17 8 24 0 100 5 10
    240606132025_d4z1 TGGGATCCTGTATAAAGGAGAAAGCAAGGCGG(+AAAAAA)GTACCAGTGTTTTTTGCTCTCTTTTCTTTAC NGG 10 8 17 8 24 0 100 5 10