Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606132008_ns14 ATCAGGTTAGCACTGGCTGTAGGCTGGTCTGG(+AAAAAA)GATCCTAACAGAATGGCAAGAGGTGAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240606132007_mtha ACTGCTTCCCTCACCTCTTGCCATTCTGTTAG(+AAAAAA)GATCCCAGACCAGCCTACAGCCAGTGCTAAC NGG 10 8 17 8 24 0 100 5 10
    240606132005_y9q8 AAAGAATTTAAAGGGGGGGGGGGGGTTTCCAG(+AAAAAA)GTACCCCTGTCCAGGCTTAAACAAAGCAAGA NGG 10 8 17 8 24 0 100 5 10
    240606132003_6nih AAGGCTCTTGCTTTGTTTAAGCCTGGACAGGG(+AAAAAA)GTACCTGGAAACCCCCCCCCCCCCTTTAAAT NGG 10 8 17 8 24 0 100 5 10
    240606132000_gav3 TTCACATCTGAGACAAATAGAAAGCAGCCCAG(+AAAAAA)GTACCAATTTGCTCTTCAGTGTCAGTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240606131959_hzx4 TTTTCCTCTGACTGACACTGAAGAGCAAATTG(+AAAAAA)GTACCTGGGCTGCTTTCTATTTGTCTCAGAT NGG 10 8 17 8 24 0 100 5 10
    240606131957_xesk GGGTGGGTGAGTGCTGGCATGGACGTCTCTAG(+AAAAAA)GATCCTCTACAAACAGGCCCAGTTCACAAGC NGG 10 8 17 8 24 0 100 5 10
    240606131956_pqop TCCTGGCTTGTGAACTGGGCCTGTTTGTAGAG(+AAAAAA)GATCCTAGAGACGTCCATGCCAGCACTCACC NGG 10 8 17 8 24 0 100 5 10
    240606131954_yk7v GTGCTGTGTTGGGAAGCTTTGGAGGTTCCTAG(+AAAAAA)GTACCTGGTATCTGGCTCCCAGCAGCAGCTC NGG 10 8 17 8 24 0 100 5 10
    240606131952_5vno AACAGGAGCTGCTGCTGGGAGCCAGATACCAG(+AAAAAA)GTACCTAGGAACCTCCAAAGCTTCCCAACAC NGG 10 8 17 8 24 0 100 5 10