Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606133355_00hv TGCTCTCTTTTAATGCAGCTTTGAAGAAGATG(+AAAAAA)GTACCAAAGGTTAAGACGGCCCCCTGATGGG NGG 10 8 17 8 24 0 100 5 10
    240606133353_eps8 TGGGCTAGGATCACTTGCCACAGAGCCCCAGG(+AAAAAA)GATCCACCTGTCTCTGTGTCCCCAGCACTGG NGG 10 8 17 8 24 0 100 5 10
    240606133351_i822 TAACCCCAGTGCTGGGGACACAGAGACAGGTG(+AAAAAA)GATCCCTGGGGCTCTGTGGCAAGTGATCCTA NGG 10 8 17 8 24 0 100 5 10
    240606133348_fxj0 AGGTGATATTAGCACAAGTTACAATTCCCTGG(+AAAAAA)GTACCAAAACCTATACATATTCTTTGGGTTT NGG 10 8 17 8 24 0 100 5 10
    240606133347_pmlt TTTTGAAACCCAAAGAATATGTATAGGTTTTG(+AAAAAA)GTACCCAGGGAATTGTAACTTGTGCTAATAT NGG 10 8 17 8 24 0 100 5 10
    240606133346_w26z TCTACAGCAAGCTCAGAGGTAGCACAAGAGAG(+AAAAAA)GATCCATCTGGTAACACCATAAATCATGCAA NGG 10 8 17 8 24 0 100 5 10
    240606133344_alox CAATTTTGCATGATTTATGGTGTTACCAGATG(+AAAAAA)GATCCTCTCTTGTGCTACCTCTGAGCTTGCT NGG 10 8 17 8 24 0 100 5 10
    240606133343_a8kh ATCATAATTCTTTTTAATTTTAAAATAGTCAG(+AAAAAA)GATCCAGTTTGAAAATATGCCACGTTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240606133342_i156 TCTCTTTTTAAACGTGGCATATTTTCAAACTG(+AAAAAA)GATCCTGACTATTTTAAAATTAAAAAGAATT NGG 10 8 17 8 24 0 100 5 10
    240606133340_1763 TGGTGTTGGACGTCGGAAGTCCTCCTGTAGTG(+AAAAAA)GATCCAAAACTCACCTCCTTATGAAAGACAA NGG 10 8 17 8 24 0 100 5 10