Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606132512_2gce TTTGTTTCGTTTGCTAATTGGTGCATATTCAG(+AAAAAA)GTACCACCTTTTGACGTGTGGATCTTTCTCC NGG 10 8 17 8 24 0 100 5 10
    240606132511_m4fo AAAAGCCCCTATGGAAAGCAAATTCCTGATTG(+AAAAAA)GTACCACCCTCGAATAGCTTATATCGTTAAA NGG 10 8 17 8 24 0 100 5 10
    240606132509_u5zo CTCTATTTAACGATATAAGCTATTCGAGGGTG(+AAAAAA)GTACCAATCAGGAATTTGCTTTCCATAGGGG NGG 10 8 17 8 24 0 100 5 10
    240606132508_sln5 GGGCCTGTTGTCCACGGAAGGCATTTGCTTAG(+AAAAAA)GATCCCCTTTGCCCCTGGCTCGGCCAGATGA NGG 10 8 17 8 24 0 100 5 10
    240606132506_ez3r ACCTTTCATCTGGCCGAGCCAGGGGCAAAGGG(+AAAAAA)GATCCTAAGCAAATGCCTTCCGTGGACAACA NGG 10 8 17 8 24 0 100 5 10
    240606132505_5iun TTACATGATAAGAAAGCTTGACACAAGCTGAG(+AAAAAA)GTACCAAGAGGCTCCTGCCACCTGGTCCACG NGG 10 8 17 8 24 0 100 5 10
    240606132503_5o4v TCACCCGTGGACCAGGTGGCAGGAGCCTCTTG(+AAAAAA)GTACCTCAGCTTGTGTCAAGCTTTCTTATCA NGG 10 8 17 8 24 0 100 5 10
    240606132502_0492 ACCCTAAACTCAAGTCAAGGTTAATGCATGCG(+AAAAAA)GATCCCGTGACGGGGCTCAGTGAGGCCCTGC NGG 10 8 17 8 24 0 100 5 10
    240606132458_2psk CCCTGGCAGGGCCTCACTGAGCCCCGTCACGG(+AAAAAA)GATCCGCATGCATTAACCTTGACTTGAGTTT NGG 10 8 17 8 24 0 100 5 10
    240606132456_casr GACTCTGGGCACGCCCTTGGCTCCCTCGGAGG(+AAAAAA)GTACCGGCTCCTCCAGAGACTAGGTTTATTT NGG 10 8 17 8 24 0 100 5 10