Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606132738_ffzt TTTCTTTTGTAAGAATGAAGAAATTTTTGTGG(+AAAAAA)GATCCAGGAAACCCACTCGGATCTCTCTCAT NGG 10 8 17 8 24 0 100 5 10
    240606132737_kwl3 AACCCTATCCATGCCATGTGGGGGGATGGCAG(+AAAAAA)GATCCGTCCTGAGGAATCTGTAGCCAACAAG NGG 10 8 17 8 24 0 100 5 10
    240606132735_cljl TAAATCTTGTTGGCTACAGATTCCTCAGGACG(+AAAAAA)GATCCTGCCATCCCCCCACATGGCATGGATA NGG 10 8 17 8 24 0 100 5 10
    240606132734_v6ti TGTATGTTAATAAGCGAAACAAAATCATACGG(+AAAAAA)GATCCAGATACATTGCATCATTGTGTTCCAT NGG 10 8 17 8 24 0 100 5 10
    240606132733_ixvd TAGAAATGGAACACAATGATGCAATGTATCTG(+AAAAAA)GATCCCGTATGATTTTGTTTCGCTTATTAAC NGG 10 8 17 8 24 0 100 5 10
    240606132731_u2zb ACCACATGGTGGCTCACAACCATCTGTAATGG(+AAAAAA)GATCCGATGCCCTCTTCTGGTGTGTCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606132729_lp6a GCTGTCTTCTGACACACCAGAAGAGGGCATCG(+AAAAAA)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606132725_wtrk CCCATAGTAATGACCCTTAAAGTTGACCTCAG(+AAAAAA)GATCCACATACCAAGAAAAGAAATTGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606132724_5iw6 CTCTAGCTCTCAATTTCTTTTCTTGGTATGTG(+AAAAAA)GATCCTGAGGTCAACTTTAAGGGTCATTACT NGG 10 8 17 8 24 0 100 5 10
    240606132722_h5ce ATTTTAAAATTAAGGCAGAGAGCACGAAGGAG(+AAAAAA)GTACCCAGGTCAGCCGACGGCCTCCACACAC NGG 10 8 17 8 24 0 100 5 10