Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606131521_26uu GAAGTGGTCTACATACTGTTGTTACTCAGGTG(+AAAAAA)GTACCAAGTGGATACCACATGCTTATGTATG NGG 10 8 17 8 24 0 100 5 10
    240606131520_gst2 GTATACATACATAAGCATGTGGTATCCACTTG(+AAAAAA)GTACCACCTGAGTAACAACAGTATGTAGACC NGG 10 8 17 8 24 0 100 5 10
    240606131518_mrkr GGGAGCATAACACCTCTGTGGTCCTTGGGGTG(+AAAAAA)GTACCTTTGAGAGAGGATTAAGGTTATTTGA NGG 10 8 17 8 24 0 100 5 10
    240606131517_9bm2 TGGCCTCAAATAACCTTAATCCTCTCTCAAAG(+AAAAAA)GTACCACCCCAAGGACCACAGAGGTGTTATG NGG 10 8 17 8 24 0 100 5 10
    240606131515_x8vl TTACAACTACAGGTTATCTTTACCTGTCCTTG(+AAAAAA)GTACCCGGTACATACCTTCCTGGTAACTATG NGG 10 8 17 8 24 0 100 5 10
    240606131513_2jiz AAATGCATAGTTACCAGGAAGGTATGTACCGG(+AAAAAA)GTACCAAGGACAGGTAAAGATAACCTGTAGT NGG 10 8 17 8 24 0 100 5 10
    240606131511_myta AATCAAACCTGGGGAGAGATCCATGCGGCACG(+AAAAAA)GATCCTGGGTTTAAGAGCAGGAAGGTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240606131509_xq93 ACTGCTTCTGACCTTCCTGCTCTTAAACCCAG(+AAAAAA)GATCCGTGCCGCATGGATCTCTCCCCAGGTT NGG 10 8 17 8 24 0 100 5 10
    240606131508_cic4 AGTGATAAAAAGAAAACAACCAAAGAAAATTG(+AAAAAA)GTACCTACTCTTCTGCATAAGAAGTGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240606131506_sne9 TATCTAGACACACTTCTTATGCAGAAGAGTAG(+AAAAAA)GTACCAATTTTCTTTGGTTGTTTTCTTTTTA NGG 10 8 17 8 24 0 100 5 10