Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606130228_t3hi GGAAGCAGGCTGGCTCCGACCCAGGGCCTCAG(+AAAAAA)GATCCCCTTGTGTACAGGGTCCATGAAACTG NGG 10 8 17 8 24 0 100 5 10
    240606130227_v9yr GTGGTCAGTTTCATGGACCCTGTACACAAGGG(+AAAAAA)GATCCTGAGGCCCTGGGTCGGAGCCAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240606130225_khlk ACTTTGAGGCAGGCACAATGCTAGGAGCGCGG(+AAAAAA)GATCCAGCAGCAGCCCCATACCCCCTCGGAT NGG 10 8 17 8 24 0 100 5 10
    240606130224_qep4 GTACGATCCGAGGGGGTATGGGGCTGCTGCTG(+AAAAAA)GATCCCGCGCTCCTAGCATTGTGCCTGCCTC NGG 10 8 17 8 24 0 100 5 10
    240606130222_7u3h CGCTCTCTGCTTTGGCTTTAAAGTCAGGCCTG(+AAAAAA)GTACCAGATAGGCAGGATCAGACTATCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606130221_keqv GATGACAAAGATAGTCTGATCCTGCCTATCTG(+AAAAAA)GTACCAGGCCTGACTTTAAAGCCAAAGCAGA NGG 10 8 17 8 24 0 100 5 10
    240606130220_bbqt TCCCTTCAACTTTCCCTAGTCTACATCTGCAG(+AAAAAA)GTACCAATAAGACCATCTACATAACCTGCAA NGG 10 8 17 8 24 0 100 5 10
    240606130219_nuog GTTTCTTGCAGGTTATGTAGATGGTCTTATTG(+AAAAAA)GTACCTGCAGATGTAGACTAGGGAAAGTTGA NGG 10 8 17 8 24 0 100 5 10
    240606130218_l7iu TCCTCCACAAATAAACGTAATTAAATCTAAAG(+AAAAAA)GTACCCTGAGATTTCTGTATAGGAAACAACT NGG 10 8 17 8 24 0 100 5 10
    240606130216_9iwa AAAACAGTTGTTTCCTATACAGAAATCTCAGG(+AAAAAA)GTACCTTTAGATTTAATTACGTTTATTTGTG NGG 10 8 17 8 24 0 100 5 10