Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606130244_uhca GGCCGGTTAGCAATCAGCGCGTTGCCTGTTGG(+AAAAAA)GTACCCTCATCTCTCAGCTGTACCCTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606130242_ye6u GGGAGCAGGGAGGGTACAGCTGAGAGATGAGG(+AAAAAA)GTACCCAACAGGCAACGCGCTGATTGCTAAC NGG 10 8 17 8 24 0 100 5 10
    240606130240_eevb AGCCCCTGAGCAAACAAGGACCTCGCGAGTGG(+AAAAAA)GTACCGAGAGGAGTGCAGGTCAGTGCTGCCA NGG 10 8 17 8 24 0 100 5 10
    240606130239_f68e CGGGGTGGCAGCACTGACCTGCACTCCTCTCG(+AAAAAA)GTACCCACTCGCGAGGTCCTTGTTTGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606130237_zpbm GACCAGATGTCAATGGCAGCTGCTTTTAGGGG(+AAAAAA)GTACCTATTCTGCTTTTAAGGGGTGCCCCCT NGG 10 8 17 8 24 0 100 5 10
    240606130236_l0tb GGGGCAGGGGGCACCCCTTAAAAGCAGAATAG(+AAAAAA)GTACCCCCTAAAAGCAGCTGCCATTGACATC NGG 10 8 17 8 24 0 100 5 10
    240606130235_aqeu ACCCTTCCAGGAGCTGCTAGTGGCAATGTCTG(+AAAAAA)GTACCCTGCCACCTTCTCCAAGCAAGGTCAG NGG 10 8 17 8 24 0 100 5 10
    240606130233_hqwp CAGCCCTGACCTTGCTTGGAGAAGGTGGCAGG(+AAAAAA)GTACCAGACATTGCCACTAGCAGCTCCTGGA NGG 10 8 17 8 24 0 100 5 10
    240606130232_rt7v ATAAGCTCCTGTCCCTGGGGACTAGGCATTAG(+AAAAAA)GATCCCAGCATACTTGGGAGGCAGGGTCATT NGG 10 8 17 8 24 0 100 5 10
    240606130231_rh2e TGTGTAATGACCCTGCCTCCCAAGTATGCTGG(+AAAAAA)GATCCTAATGCCTAGTCCCCAGGGACAGGAG NGG 10 8 17 8 24 0 100 5 10