Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125706_0ih1 CCTGTGTCTCAGGGACGCCTCTACTTTCTTGG(+AAAAAA)GATCCGGGACCGTTCTCAGAGGCACTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240606125703_t6fr TTCCGTCCCCAGTGCCTCTGAGAACGGTCCCG(+AAAAAA)GATCCCAAGAAAGTAGAGGCGTCCCTGAGAC NGG 10 8 17 8 24 0 100 5 10
    240606125702_7zbs CAGCAAGATGCTCCTGATGCCCTTTGGTCCAG(+AAAAAA)GATCCACTGCCTCCTTGTTCTAGAGGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606125700_ggvq GATGTCTGAGCCTCTAGAACAAGGAGGCAGTG(+AAAAAA)GATCCTGGACCAAAGGGCATCAGGAGCATCT NGG 10 8 17 8 24 0 100 5 10
    240606125659_wldq CCGCATGATGGCTGGGTCTGAAATCGCAAATG(+AAAAAA)GATCCAGCCTGATGTCCTCGCAGTTGCACAT NGG 10 8 17 8 24 0 100 5 10
    240606125658_darm CCCACATGTGCAACTGCGAGGACATCAGGCTG(+AAAAAA)GATCCATTTGCGATTTCAGACCCAGCCATCA NGG 10 8 17 8 24 0 100 5 10
    240606125656_18gz ATAGATACACTGACTTGGCTTCCTGGGCTGGG(+AAAAAA)GTACCAGGAAGGAAGAAACCAGACAGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240606125654_k8qt AGGGAAAAACCTGTCTGGTTTCTTCCTTCCTG(+AAAAAA)GTACCCCAGCCCAGGAAGCCAAGTCAGTGTA NGG 10 8 17 8 24 0 100 5 10
    240606125653_i8ze GAGAACTCAGCTGGCTTTCTGGGTTTTCTCAG(+AAAAAA)GTACCTGTTTCCTCAGGGCTGTTGTTCCCAC NGG 10 8 17 8 24 0 100 5 10
    240606125652_amzu CCTCTGTGGGAACAACAGCCCTGAGGAAACAG(+AAAAAA)GTACCTGAGAAAACCCAGAAAGCCAGCTGAG NGG 10 8 17 8 24 0 100 5 10