Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125602_rmn6 CAGCTTGGGCCAGCTGCTCTGAGCCTCCAAGG(+AAAAAA)GATCCCCCGCCCCCTCCCCTGGAATGAAATC NGG 10 8 17 8 24 0 100 5 10
    240606125600_kx8y GAAATGATTTCATTCCAGGGGAGGGGGCGGGG(+AAAAAA)GATCCCTTGGAGGCTCAGAGCAGCTGGCCCA NGG 10 8 17 8 24 0 100 5 10
    240606125558_eik3 CATCAGAGGAGAGTAAAGCAGGGACAACAGGG(+AAAAAA)GTACCCAGCCTAGATCTTGTTGCTGAATCCA NGG 10 8 17 8 24 0 100 5 10
    240606125556_legl ACCACTGGATTCAGCAACAAGATCTAGGCTGG(+AAAAAA)GTACCCCTGTTGTCCCTGCTTTACTCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240606125555_vshm TAGCAAACTCCCCAGTCCCCACAGCCACTCGG(+AAAAAA)GTACCTCTTTTCCATAGACAGTTCTGATCAC NGG 10 8 17 8 24 0 100 5 10
    240606125553_wue0 CACACGTGATCAGAACTGTCTATGGAAAAGAG(+AAAAAA)GTACCCGAGTGGCTGTGGGGACTGGGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240606125552_3coe TCTGGCATTTCATGTTACTGCAGGCCTTTGAG(+AAAAAA)GTACCTCAATGTGTAAGATATTTCTGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606125551_6fw0 TTAAGCCCCACAGAAATATCTTACACATTGAG(+AAAAAA)GTACCTCAAAGGCCTGCAGTAACATGAAATG NGG 10 8 17 8 24 0 100 5 10
    240606125550_bhb6 ATACTGAAACAGACTTCCAACAGGAAGGCACG(+AAAAAA)GTACCTGAGAGCAAGTGTTTCTGAATAGAAC NGG 10 8 17 8 24 0 100 5 10
    240606125548_miol GTCCAGTTCTATTCAGAAACACTTGCTCTCAG(+AAAAAA)GTACCGTGCCTTCCTGTTGGAAGTCTGTTTC NGG 10 8 17 8 24 0 100 5 10