Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125458_g8sj TCCTCACTCATTTTCAACGCTGACCCTGACAG(+AAAAAA)GATCCACAGACTTCTCCAGGACCTTCCAGAG NGG 10 8 17 8 24 0 100 5 10
    240606125457_kt2p AGGGTCTCTGGAAGGTCCTGGAGAAGTCTGTG(+AAAAAA)GATCCTGTCAGGGTCAGCGTTGAAAATGAGT NGG 10 8 17 8 24 0 100 5 10
    240606125456_7vao TCCCCCAGGGGAGAGGCCCCTTTACCTCAGTG(+AAAAAA)GATCCTGTTAAGGAGTGCTTCCAGCCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240606125454_kd5e CCCAGGCTGAGGCTGGAAGCACTCCTTAACAG(+AAAAAA)GATCCACTGAGGTAAAGGGGCCTCTCCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606125453_hs28 CCTACAGTGTTACTTCCTAGGGGCTACGCAGG(+AAAAAA)GATCCGTTTCTCTCCACTGTGCTATTCACAA NGG 10 8 17 8 24 0 100 5 10
    240606125451_iluj CAACGTTGTGAATAGCACAGTGGAGAGAAACG(+AAAAAA)GATCCCTGCGTAGCCCCTAGGAAGTAACACT NGG 10 8 17 8 24 0 100 5 10
    240606125450_r2co AGAAGAAAGCAATCCCTGAAATCTGGCAGGCG(+AAAAAA)GTACCAGAGGCCACAGTTGTTCTGGTGAGTG NGG 10 8 17 8 24 0 100 5 10
    240606125448_igik TACCACACTCACCAGAACAACTGTGGCCTCTG(+AAAAAA)GTACCGCCTGCCAGATTTCAGGGATTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240606125446_uu1f GGGGGTCACCCTATACACTAAGGCCTGTGGGG(+AAAAAA)GTACCCACCCTATACACTAAGGCCTGTGCTC NGG 10 8 17 8 24 0 100 5 10
    240606125444_ify5 GCCTGGAGCACAGGCCTTAGTGTATAGGGTGG(+AAAAAA)GTACCCCCACAGGCCTTAGTGTATAGGGTGA NGG 10 8 17 8 24 0 100 5 10