Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125428_vdv8 TTAAAATCTAATACCTGTAGCACAAAGTTGTG(+AAAAAA)GATCCATATAGCAGAAATTGGCTGCCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606125426_fr7d GGGGTCAAAGGGCAGCCAATTTCTGCTATATG(+AAAAAA)GATCCACAACTTTGTGCTACAGGTATTAGAT NGG 10 8 17 8 24 0 100 5 10
    240606125425_kb0x CCACATGGTGGTTCACAACCATCTGTAATGGG(+AAAAAA)GATCCATATACATAAATAAACCTTTTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606125423_sxct AATATTTTTTAAAAGGTTTATTTATGTATATG(+AAAAAA)GATCCCCATTACAGATGGTTGTGAACCACCA NGG 10 8 17 8 24 0 100 5 10
    240606125422_n2bt TTAAAATCTAATACCTGTAGCACAAAGTTGTG(+AAAAAA)GATCCATATAGCAGAAATTGGCTGCCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606125421_1guw GGGGTCAAAGGGCAGCCAATTTCTGCTATATG(+AAAAAA)GATCCACAACTTTGTGCTACAGGTATTAGAT NGG 10 8 17 8 24 0 100 5 10
    240606125419_uzcz CTAGGGAGCCATCTGGGATGTTGGCATGTTGG(+AAAAAA)GTACCTGTCCTGTGACACTTGATTGTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240606125418_hq5u CCACCCAAGCACAATCAAGTGTCACAGGACAG(+AAAAAA)GTACCCAACATGCCAACATCCCAGATGGCTC NGG 10 8 17 8 24 0 100 5 10
    240606125417_1iom GGAGAGTCACTGGCCTCTCCCGTTGGTTACAG(+AAAAAA)GTACCCTTATACCCTGGATGTTAGCGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240606125415_n3lz ACAACTACAACGCTAACATCCAGGGTATAAGG(+AAAAAA)GTACCTGTAACCAACGGGAGAGGCCAGTGAC NGG 10 8 17 8 24 0 100 5 10