Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125321_getx CTTAGTCCTTAGACTTGATGTGCCATGGATTG(+AAAAAA)GTACCATGGGTGGTAGTGGGGGTGGTGACAG NGG 10 8 17 8 24 0 100 5 10
    240606125318_0ur6 GAGCCCTGTCACCACCCCCACTACCACCCATG(+AAAAAA)GTACCAATCCATGGCACATCAAGTCTAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606125316_tlan CAGTCAGGGACAAGGCTGTCTTCAGGTGTGGG(+AAAAAA)GATCCTGGGACTCCAACATGCAGGAAGGGGC NGG 10 8 17 8 24 0 100 5 10
    240606125313_0ulu GTAGAGCCCCTTCCTGCATGTTGGAGTCCCAG(+AAAAAA)GATCCCCACACCTGAAGACAGCCTTGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240606125311_68hw GCTCACAATGGCCTACAACTCCAGTTCCTGAG(+AAAAAA)GATCCAGTACCCTTACATTCACACACATGAA NGG 10 8 17 8 24 0 100 5 10
    240606125310_jpp1 TTGCCTTCATGTGTGTGAATGTAAGGGTACTG(+AAAAAA)GATCCTCAGGAACTGGAGTTGTAGGCCATTG NGG 10 8 17 8 24 0 100 5 10
    240606125308_v4jl CTTATCTTCAGACACACTAGAAGAGGCCATCG(+AAAAAA)GATCCTATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606125307_v3j0 ATCACATGGTGGCTCACAACCATCTGTAATAG(+AAAAAA)GATCCGATGGCCTCTTCTAGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606125305_w0uw CCCTTCCCACTGAGATGCCTGGGCAGCCATGG(+AAAAAA)GTACCCCGAGGCTGATAACACATCAGAGGTG NGG 10 8 17 8 24 0 100 5 10
    240606125303_y0x9 AATAGCACCTCTGATGTGTTATCAGCCTCGGG(+AAAAAA)GTACCCATGGCTGCCCAGGCATCTCAGTGGG NGG 10 8 17 8 24 0 100 5 10