Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125301_bb5i GAACCCAGAAGGAGGGTCCAGTGGGCTCTTTG(+AAAAAA)GATCCTTCAGGCTCTTGGAGACGAGAAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606125300_ab4f CTCTCCAGCTTCTCGTCTCCAAGAGCCTGAAG(+AAAAAA)GATCCAAAGAGCCCACTGGACCCTCCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240606125258_fize CTACATTCAGGAATATATATACACATTCCTGG(+AAAAAA)GTACCTAGCCACACATGCATACATAAACACA NGG 10 8 17 8 24 0 100 5 10
    240606125256_dzuc GCATGTGTGTTTATGTATGCATGTGTGGCTAG(+AAAAAA)GTACCCAGGAATGTGTATATATATTCCTGAA NGG 10 8 17 8 24 0 100 5 10
    240606125255_r2nl CTCCTCCTTTGAGTCATTGCTTCACCCACTAG(+AAAAAA)GATCCTGCCTTACCTTGACACCCAGAAACAA NGG 10 8 17 8 24 0 100 5 10
    240606125254_iy03 TTGTTTTGTTTCTGGGTGTCAAGGTAAGGCAG(+AAAAAA)GATCCTAGTGGGTGAAGCAATGACTCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240606125252_vnsj AGAAAGGGAGGGATAACCTCTTTCTCAACTAG(+AAAAAA)GTACCAGCTTGAGTCCTTATGTCAAAGGCGA NGG 10 8 17 8 24 0 100 5 10
    240606125251_0t9n CTTCATCGCCTTTGACATAAGGACTCAAGCTG(+AAAAAA)GTACCTAGTTGAGAAAGAGGTTATCCCTCCC NGG 10 8 17 8 24 0 100 5 10
    240606125249_r2uq ACACTGAGAGAGCAGCAATAACTATCACCTCG(+AAAAAA)GATCCAGAGTAACACTGAAGTACACGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240606125248_msp2 GGCTGGCCTCCGTGTACTTCAGTGTTACTCTG(+AAAAAA)GATCCGAGGTGATAGTTATTGCTGCTCTCTC NGG 10 8 17 8 24 0 100 5 10