Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125159_six8 GGAGAGGATAACCAAAGCCTGGCTACAACTGG(+AAAAAA)GTACCAGAATGCCTGCCGACCACCTGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240606125157_i6p1 GGTGAGGCAGCAGGTGGTCGGCAGGCATTCTG(+AAAAAA)GTACCCAGTTGTAGCCAGGCTTTGGTTATCC NGG 10 8 17 8 24 0 100 5 10
    240606125156_dxu1 TGAACTCTTGGTTTGGGAAAGAGGAGAGTCAG(+AAAAAA)GATCCTCCACTCAGGGGCCTGCTTGGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240606125155_mfch AGGGCTGCAGCCAAGCAGGCCCCTGAGTGGAG(+AAAAAA)GATCCTGACTCTCCTCTTTCCCAAACCAAGA NGG 10 8 17 8 24 0 100 5 10
    240606125152_ddod GTTACCTTTCGCTGTGGGGAGGCCGCTGCCGG(+AAAAAA)GATCCGGCCCCGAACAGCCCAGGGGCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240606125150_zquz CCCCGCCCCTGCCCCTGGGCTGTTCGGGGCCG(+AAAAAA)GATCCCGGCAGCGGCCTCCCCACAGCGAAAG NGG 10 8 17 8 24 0 100 5 10
    240606125149_bk5r CGGGGTCCTGCCCTACCCAGGCTATGCTACAG(+AAAAAA)GATCCACCACAGTCCAAAACCAGGAATTAGA NGG 10 8 17 8 24 0 100 5 10
    240606125148_tl6x CTCTCTCTAATTCCTGGTTTTGGACTGTGGTG(+AAAAAA)GATCCTGTAGCATAGCCTGGGTAGGGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240606125146_97n1 AGATGTATCCTGAAGCCTTCTTGCCTATCTGG(+AAAAAA)GTACCAGGTGTGACCTCAAGAGATGGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240606125144_wgeu TGTACAGCAGCCATCTCTTGAGGTCACACCTG(+AAAAAA)GTACCCAGATAGGCAAGAAGGCTTCAGGATA NGG 10 8 17 8 24 0 100 5 10