Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125214_s1kc GAGCTCAGCACCACTTGCCTGCTAGGGCCTTG(+AAAAAA)GTACCAGTGTGCAGATAGACCTAGCCAAGGC NGG 10 8 17 8 24 0 100 5 10
    240606125213_7sfh CCTGTGCCTTGGCTAGGTCTATCTGCACACTG(+AAAAAA)GTACCAAGGCCCTAGCAGGCAAGTGGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606125212_d2w6 TGTTGAGAGGAAAAATGAAATGGGACAACGTG(+AAAAAA)GTACCAACCAGCTCAGGGCCCACTAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606125210_c8es GTGCTCTTCCTTAGTGGGCCCTGAGCTGGTTG(+AAAAAA)GTACCACGTTGTCCCATTTCATTTTTCCTCT NGG 10 8 17 8 24 0 100 5 10
    240606125208_kcit CACAATCCTGCCCATTCCCCTGTGCCTGGGGG(+AAAAAA)GATCCCCTCCAGAGGTGTTCTCTATCCCGCC NGG 10 8 17 8 24 0 100 5 10
    240606125206_w0f3 GGGTAGGCGGGATAGAGAACACCTCTGGAGGG(+AAAAAA)GATCCCCCCAGGCACAGGGGAATGGGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240606125204_prpp TTTGAGCAAGGTTTCCTTCCCAAAGCTTACAG(+AAAAAA)GATCCTGCTTTTGGTATAACTGTAGCTAGCA NGG 10 8 17 8 24 0 100 5 10
    240606125203_0qy0 CGAGGTGCTAGCTACAGTTATACCAAAAGCAG(+AAAAAA)GATCCTGTAAGCTTTGGGAAGGAAACCTTGC NGG 10 8 17 8 24 0 100 5 10
    240606125202_5jlt TTACTCACAAATAAACTAATGCTACTTGCAAG(+AAAAAA)GATCCTTGGTAAAGTAAAACCTGATGAATAT NGG 10 8 17 8 24 0 100 5 10
    240606125200_6cgl TGAAAATATTCATCAGGTTTTACTTTACCAAG(+AAAAAA)GATCCTTGCAAGTAGCATTAGTTTATTTGTG NGG 10 8 17 8 24 0 100 5 10