Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125143_ojin GGTGACCTCAAAGGTATCCCATGATCAGCATG(+AAAAAA)GTACCAGCAGTAGTGAGTAACCAGTTCTAGT NGG 10 8 17 8 24 0 100 5 10
    240606125142_xjxz GGGAAACTAGAACTGGTTACTCACTACTGCTG(+AAAAAA)GTACCATGCTGATCATGGGATACCTTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240606125141_wxlx ACGTGATGGTAGGCTTGTGGGGTGACTGCTAG(+AAAAAA)GTACCTCTAGAGGGCAACACTTACACAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606125139_ic9l CTGTTTTCCTGTGTAAGTGTTGCCCTCTAGAG(+AAAAAA)GTACCTAGCAGTCACCCCACAAGCCTACCAT NGG 10 8 17 8 24 0 100 5 10
    240606125137_38ou CAGGTGGTCTGACATCCTCTTGTGCCTCCCTG(+AAAAAA)GTACCTGCAGACACATTCTCTCTCTCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240606125136_ufo2 GAGAGAGACAGAGAGAGAGAATGTGTCTGCAG(+AAAAAA)GTACCAGGGAGGCACAAGAGGATGTCAGACC NGG 10 8 17 8 24 0 100 5 10
    240606125134_sp9i GTGTGCACTGCTTGTATGCGTGTGGATGTGTG(+AAAAAA)GATCCCACCTGCCGCCTCCCCATCTGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240606125133_emto ACACCCAGAGCAGATGGGGAGGCGGCAGGTGG(+AAAAAA)GATCCACACATCCACACGCATACAAGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240606125131_ea85 TCTGGGTGGGCGAGATACCCAATGCCAGCCAG(+AAAAAA)GTACCAGCGCCCCAGGAGCATGAAATGCCAG NGG 10 8 17 8 24 0 100 5 10
    240606125129_iu8s GTGGCCTGGCATTTCATGCTCCTGGGGCGCTG(+AAAAAA)GTACCTGGCTGGCATTGGGTATCTCGCCCAC NGG 10 8 17 8 24 0 100 5 10