Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606130500_7z4f ATGGTAATAAATAAATAAGATTTCTTTTAATG(+AAAAAA)GATCCAGACTTACCTGAGGACAACTGTAGGG NGG 10 8 17 8 24 0 100 5 10
    240606130459_ysho ATTAACCCTACAGTTGTCCTCAGGTAAGTCTG(+AAAAAA)GATCCATTAAAAGAAATCTTATTTATTTATT NGG 10 8 17 8 24 0 100 5 10
    240606130457_j0ol GATCAGCATCCAGGCCCATCCATCAGCCTTCG(+AAAAAA)GATCCTGCTCTGCTCTTTATTCTGCTGTCTC NGG 10 8 17 8 24 0 100 5 10
    240606130456_mgmo GGAGTGAGACAGCAGAATAAAGAGCAGAGCAG(+AAAAAA)GATCCGAAGGCTGATGGATGGGCCTGGATGC NGG 10 8 17 8 24 0 100 5 10
    240606130454_ndsd AACCTCTGGGTTTGGTGGCTGATTATGGGATG(+AAAAAA)GATCCCCAGGTGGGGTAGTCTCTGGATAGTC NGG 10 8 17 8 24 0 100 5 10
    240606130452_qkx0 GGATGGACTATCCAGAGACTACCCCACCTGGG(+AAAAAA)GATCCATCCCATAATCAGCCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606130450_y0bn AATAGAAAAAAAAGTAAAACCTCACTTCACAG(+AAAAAA)GATCCCCCTCCATTCCCTATGTTCCTGTAAA NGG 10 8 17 8 24 0 100 5 10
    240606130448_dcov ACACCTTTACAGGAACATAGGGAATGGAGGGG(+AAAAAA)GATCCTGTGAAGTGAGGTTTTACTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240606130447_zzh4 GTGCTTTGGTTGTATGATGGGGGAGTGGGCAG(+AAAAAA)GTACCCACTTGCTGCCTCTGTCCTCATCTAT NGG 10 8 17 8 24 0 100 5 10
    240606130445_06m8 TCTGGATAGATGAGGACAGAGGCAGCAAGTGG(+AAAAAA)GTACCTGCCCACTCCCCCATCATACAACCAA NGG 10 8 17 8 24 0 100 5 10