Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125231_eqdm GATCTATCTTGTTATGCAGCCAGAATGGCCTG(+AAAAAA)GATCCCCCTCCTGTGTTTTCAGGTGTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240606125229_hef4 CAAAAGAGCAACACCTGAAAACACAGGAGGGG(+AAAAAA)GATCCAGGCCATTCTGGCTGCATAACAAGAT NGG 10 8 17 8 24 0 100 5 10
    240606125227_6k4x CCCTCCCATAGCAGGCATGCTGACACATCCTG(+AAAAAA)GTACCCTGCCGTACTAACTGCTCTGCTGCCT NGG 10 8 17 8 24 0 100 5 10
    240606125225_jepj CGTTGAGGCAGCAGAGCAGTTAGTACGGCAGG(+AAAAAA)GTACCAGGATGTGTCAGCATGCCTGCTATGG NGG 10 8 17 8 24 0 100 5 10
    240606125223_j495 CACCTTGCATATGTGAGGCCTTTGCCACCCAG(+AAAAAA)GATCCTCTGACATCTGGGTGATTTCAGATGC NGG 10 8 17 8 24 0 100 5 10
    240606125221_49wf AAGCTGCATCTGAAATCACCCAGATGTCAGAG(+AAAAAA)GATCCTGGGTGGCAAAGGCCTCACATATGCA NGG 10 8 17 8 24 0 100 5 10
    240606125220_2jyd GATGAGAACGGAGTTGACAATGGTTTCATGAG(+AAAAAA)GATCCTGTCAGTAGGGGTGATTTAAGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240606125219_zwi3 TCCTTCCTTCCTTAAATCACCCCTACTGACAG(+AAAAAA)GATCCTCATGAAACCATTGTCAACTCCGTTC NGG 10 8 17 8 24 0 100 5 10
    240606125218_y8n7 CCCTAGATAGCTATAAAGGAATAGCTAAGGAG(+AAAAAA)GTACCCACATCTCACATGTAGTTCTAGATGA NGG 10 8 17 8 24 0 100 5 10
    240606125216_652o ATAATTCATCTAGAACTACATGTGAGATGTGG(+AAAAAA)GTACCTCCTTAGCTATTCCTTTATAGCTATC NGG 10 8 17 8 24 0 100 5 10