Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125354_mjth CCTCTTCCCTCTGCCCAGCACCTAAGAGCAGG(+AAAAAA)GATCCCACGGCGGGGAGATGAGCAGGGGTGC NGG 10 8 17 8 24 0 100 5 10
    240606125352_jmm8 AGACCGCACCCCTGCTCATCTCCCCGCCGTGG(+AAAAAA)GATCCCTGCTCTTAGGTGCTGGGCAGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240606125350_7t2y TCCTAAGGGAGCCAGATGTCAAGTCCAGGGTG(+AAAAAA)GTACCCGATAACAATCTCCTGGGCTTCAGGT NGG 10 8 17 8 24 0 100 5 10
    240606125348_mow9 TCAGGACCTGAAGCCCAGGAGATTGTTATCGG(+AAAAAA)GTACCACCCTGGACTTGACATCTGGCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240606125346_b2lm GTCAACTAAGGCGGCTATTGTTTGTGCCTCAG(+AAAAAA)GATCCTAAGGGAGCCAGATGTCAAGTCCAGG NGG 10 8 17 8 24 0 100 5 10
    240606125344_u67t ACCACCCTGGACTTGACATCTGGCTCCCTTAG(+AAAAAA)GATCCTGAGGCACAAACAATAGCCGCCTTAG NGG 10 8 17 8 24 0 100 5 10
    240606125343_exwv TCCAGCTTCTGCCAAGCTCCCCTAAGGAATTG(+AAAAAA)GATCCAAAGTAAAATGGCCCATAGCTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606125342_qz6u TGACCAGGAGAGCTATGGGCCATTTTACTTTG(+AAAAAA)GATCCAATTCCTTAGGGGAGCTTGGCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606125339_772i GAGGAGGTGTAGACTGGGAACTAGTGCCTGGG(+AAAAAA)GTACCCCATCTTGAGGTCAGCAGCAAGTGGG NGG 10 8 17 8 24 0 100 5 10
    240606125337_xosm GTGGCCCCACTTGCTGCTGACCTCAAGATGGG(+AAAAAA)GTACCCCAGGCACTAGTTCCCAGTCTACACC NGG 10 8 17 8 24 0 100 5 10