Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125530_7e3z GAGGCATGTCAGCTACCAGTTTCTCCACAGAG(+AAAAAA)GATCCAAGAAGTACATCAAAGATGGTCTACC NGG 10 8 17 8 24 0 100 5 10
    240606125529_oku5 GAGATGGTAGACCATCTTTGATGTACTTCTTG(+AAAAAA)GATCCTCTGTGGAGAAACTGGTAGCTGACAT NGG 10 8 17 8 24 0 100 5 10
    240606125528_5uiy CAGTGAGTGCCAGCGGTGCTGGAGCTAGAGCG(+AAAAAA)GATCCTGAAGGCAGTGACATTGGGACAGTCA NGG 10 8 17 8 24 0 100 5 10
    240606125526_ai4t GACCCTGACTGTCCCAATGTCACTGCCTTCAG(+AAAAAA)GATCCGCTCTAGCTCCAGCACCGCTGGCACT NGG 10 8 17 8 24 0 100 5 10
    240606125525_vska TAAGAAGAGTCTCCCCTGAAGTGTTTGTGGTG(+AAAAAA)GTACCTGTGTGCAGAAGGGTGTGTCGCCACT NGG 10 8 17 8 24 0 100 5 10
    240606125524_o0nh TGCCAAGTGGCGACACACCCTTCTGCACACAG(+AAAAAA)GTACCACCACAAACACTTCAGGGGAGACTCT NGG 10 8 17 8 24 0 100 5 10
    240606125522_iyv3 TGAGATGTCCTGACTAATCTTCCAGAACACAG(+AAAAAA)GATCCAGCAGGGAAAAGATTCACCAGCTAAT NGG 10 8 17 8 24 0 100 5 10
    240606125521_e8n2 AAGAAATTAGCTGGTGAATCTTTTCCCTGCTG(+AAAAAA)GATCCTGTGTTCTGGAAGATTAGTCAGGACA NGG 10 8 17 8 24 0 100 5 10
    240606125520_kmu1 GGCATGGGTCCGAAGCTCCTTCAATCCTGGTG(+AAAAAA)GATCCGTGGGGAAAGGACGGGGATGGTGAAA NGG 10 8 17 8 24 0 100 5 10
    240606125517_id6l CTTGGTTTCACCATCCCCGTCCTTTCCCCACG(+AAAAAA)GATCCACCAGGATTGAAGGAGCTTCGGACCC NGG 10 8 17 8 24 0 100 5 10