Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125546_9530 TAAATGACAGTGTGTAACTATCTGTAACTGGG(+AAAAAA)GATCCCATGCCCTTTTCTGACCTTCAAGGTC NGG 10 8 17 8 24 0 100 5 10
    240606125545_qyp9 TTGGTGACCTTGAAGGTCAGAAAAGGGCATGG(+AAAAAA)GATCCCCAGTTACAGATAGTTACACACTGTC NGG 10 8 17 8 24 0 100 5 10
    240606125543_ooce GCCGTTAGACTGCCTGCCTTTCAGAGGTCTGG(+AAAAAA)GATCCTGGCTCCCCACCCCCAAGTGCGAGAC NGG 10 8 17 8 24 0 100 5 10
    240606125541_t1hk AAATGGTCTCGCACTTGGGGGTGGGGAGCCAG(+AAAAAA)GATCCCAGACCTCTGAAAGGCAGGCAGTCTA NGG 10 8 17 8 24 0 100 5 10
    240606125540_k6fz TTAGGAACAAGGCAGAGAGCACAGTCGCTCTG(+AAAAAA)GATCCACCCTAAAAAGCCTGCTGACGTGGAG NGG 10 8 17 8 24 0 100 5 10
    240606125538_gfm3 TGTCCCTCCACGTCAGCAGGCTTTTTAGGGTG(+AAAAAA)GATCCAGAGCGACTGTGCTCTCTGCCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240606125537_6ph3 TCCTCATGTAGCTCACAAGGGCTGGTGTGGAG(+AAAAAA)GATCCGCCTTCTTGCTGGAGAACCCAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240606125535_pjfy TAGGGGAGGCTGGGTTCTCCAGCAAGAAGGCG(+AAAAAA)GATCCTCCACACCAGCCCTTGTGAGCTACAT NGG 10 8 17 8 24 0 100 5 10
    240606125534_w7g6 GGCAGAGATGGCTCCATGTTTTACAAACTATG(+AAAAAA)GATCCCTGGGCAGAGTTCAGGTGTCCCTGTA NGG 10 8 17 8 24 0 100 5 10
    240606125531_pl6e AAGGATACAGGGACACCTGAACTCTGCCCAGG(+AAAAAA)GATCCATAGTTTGTAAAACATGGAGCCATCT NGG 10 8 17 8 24 0 100 5 10