Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125753_xajo GAGTCAGTTCTTGAGCCCAGCTTTCCAGTGAG(+AAAAAA)GATCCGCACCTGCGGGATGTGTGTCCTATTC NGG 10 8 17 8 24 0 100 5 10
    240606125752_af24 CACATGAATAGGACACACATCCCGCAGGTGCG(+AAAAAA)GATCCTCACTGGAAAGCTGGGCTCAAGAACT NGG 10 8 17 8 24 0 100 5 10
    240606125750_pok3 AATAATGCTCACTTCCCAGTCTGTGGCCACGG(+AAAAAA)GATCCCATGTGTCTTTTTGCTTGTGGCAGAT NGG 10 8 17 8 24 0 100 5 10
    240606125748_ugs7 AAGAAATCTGCCACAAGCAAAAAGACACATGG(+AAAAAA)GATCCCGTGGCCACAGACTGGGAAGTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240606125747_0csd AGGGCAGGACCTGGTCCATCTCTGCGGGACTG(+AAAAAA)GTACCAACCAATAAGGCAGAGGGGGTTGAGC NGG 10 8 17 8 24 0 100 5 10
    240606125745_8i3v GGCGAGCTCAACCCCCTCTGCCTTATTGGTTG(+AAAAAA)GTACCAGTCCCGCAGAGATGGACCAGGTCCT NGG 10 8 17 8 24 0 100 5 10
    240606125744_6iin GGTGTCAGCAGTTGGAGCAGCCTGGAGTTCTG(+AAAAAA)GATCCTGGCTCCTAGGTGGTTTTCACATCAG NGG 10 8 17 8 24 0 100 5 10
    240606125742_b7fl AGAGGCTGATGTGAAAACCACCTAGGAGCCAG(+AAAAAA)GATCCAGAACTCCAGGCTGCTCCAACTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606125740_p3q3 TGTGTATGCAAAAACTCCTGTCCACATGGTGG(+AAAAAA)GTACCCCACGGAAGACAGCTTTCTTATTTGA NGG 10 8 17 8 24 0 100 5 10
    240606125739_x1ay GTTTTTCAAATAAGAAAGCTGTCTTCCGTGGG(+AAAAAA)GTACCCACCATGTGGACAGGAGTTTTTGCAT NGG 10 8 17 8 24 0 100 5 10