Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125905_1srf TCTGTTCAATAGAGGCCACTTCCTTCCCAAGG(+AAAAAA)GATCCAAAACCCAAAACCTGCGGCTTCCACT NGG 10 8 17 8 24 0 100 5 10
    240606125904_gqjq GTTTCAGTGGAAGCCGCAGGTTTTGGGTTTTG(+AAAAAA)GATCCCTTGGGAAGGAAGTGGCCTCTATTGA NGG 10 8 17 8 24 0 100 5 10
    240606125902_0ed3 AAGTCCATTTCCCAGCCATACGCAGCTGTGGG(+AAAAAA)GATCCCAGGAGACACTTCAAGTGGAATGAAT NGG 10 8 17 8 24 0 100 5 10
    240606125859_8rgb TTGAGATTCATTCCACTTGAAGTGTCTCCTGG(+AAAAAA)GATCCCCACAGCTGCGTATGGCTGGGAAATG NGG 10 8 17 8 24 0 100 5 10
    240606125858_uyza TGGAATGGCTGTCCTGTAGGCAGGTTGGGGTG(+AAAAAA)GTACCCTGGGGCTTCTGGGAATGGGGCTATG NGG 10 8 17 8 24 0 100 5 10
    240606125855_u08j AACACCATAGCCCCATTCCCAGAAGCCCCAGG(+AAAAAA)GTACCACCCCAACCTGCCTACAGGACAGCCA NGG 10 8 17 8 24 0 100 5 10
    240606125853_swni GGGGGGCTCTCATCCCCTCCTCCACCCCAGAG(+AAAAAA)GATCCAAGTGAGCACCAGCTGACCTGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606125852_74qa CGGGACTGAGCAGGTCAGCTGGTGCTCACTTG(+AAAAAA)GATCCTCTGGGGTGGAGGAGGGGATGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606125849_mdaj TGCTTCAAGCTGTGAGGAAGCCGCAGCCCAGG(+AAAAAA)GATCCCAAGCTTTTCGGGAGTGGCCTTGTTC NGG 10 8 17 8 24 0 100 5 10
    240606125847_r27z CCACTGAACAAGGCCACTCCCGAAAAGCTTGG(+AAAAAA)GATCCCTGGGCTGCGGCTTCCTCACAGCTTG NGG 10 8 17 8 24 0 100 5 10