Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125938_rcur TTGTCCCCAGGATACAGCAGATGCTCAACAGG(+AAAAAA)GTACCAGAGACACTGTCTGTCTGGAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606125937_kt13 CCGCCAGAGCCTCCAGACAGACAGTGTCTCTG(+AAAAAA)GTACCCTGTTGAGCATCTGCTGTATCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606125935_eigt TTTTCCTTTCCAGAAACATGTTTGGGGCCGTG(+AAAAAA)GATCCGAACAGGCAACAGGGGTGCTCTGCCA NGG 10 8 17 8 24 0 100 5 10
    240606125934_27gr ATCTTTGGCAGAGCACCCCTGTTGCCTGTTCG(+AAAAAA)GATCCACGGCCCCAAACATGTTTCTGGAAAG NGG 10 8 17 8 24 0 100 5 10
    240606125933_fjce CAAGGAAGGGCCCTCTGTCCCCTGATATGCTG(+AAAAAA)GTACCATTGTTTGTGTCCAGGGGATGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606125931_yq0p GGTGAGCTGGCATCCCCTGGACACAAACAATG(+AAAAAA)GTACCAGCATATCAGGGGACAGAGGGCCCTT NGG 10 8 17 8 24 0 100 5 10
    240606125930_16mn CAGGCCAGGACCCACACAACTGATGGGGGCAG(+AAAAAA)GATCCCCAGTGATGGGTCCAGAGTGGGAATC NGG 10 8 17 8 24 0 100 5 10
    240606125928_4lp6 GACTTGATTCCCACTCTGGACCCATCACTGGG(+AAAAAA)GATCCTGCCCCCATCAGTTGTGTGGGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240606125926_wm7j GGTCTCCCGAGAGCATGCCGAGTGTCCTGAGG(+AAAAAA)GATCCTCGGCTAGCCCCCATCTTCTACTTGA NGG 10 8 17 8 24 0 100 5 10
    240606125925_xgef AGCCTTCAAGTAGAAGATGGGGGCTAGCCGAG(+AAAAAA)GATCCCTCAGGACACTCGGCATGCTCTCGGG NGG 10 8 17 8 24 0 100 5 10