Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606130009_bsrs CATCCAGGCCCCCAATCTTTTATATTGAAGGG(+AAAAAA)GATCCAACTTCCAGGATGAAAGGCAGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606130008_rs5q ACCAGGCTCTCTGCCTTTCATCCTGGAAGTTG(+AAAAAA)GATCCCCTTCAATATAAAAGATTGGGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240606130007_5i2w GCTGAGCAAGAAAGGCTGTCTGAGCCATGGTG(+AAAAAA)GATCCCCAGGGAGGGAATGTCCCCTCCCCCC NGG 10 8 17 8 24 0 100 5 10
    240606130004_1fdp GGGCAGGGGGGAGGGGACATTCCCTCCCTGGG(+AAAAAA)GATCCACCATGGCTCAGACAGCCTTTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240606130003_jnqb GCCTACTTCTGGATCACCCTCATCCAGTGCTG(+AAAAAA)GTACCCCACAGCACTAAATCCAGGAACCCTG NGG 10 8 17 8 24 0 100 5 10
    240606130001_b2v6 AAGCCCAGGGTTCCTGGATTTAGTGCTGTGGG(+AAAAAA)GTACCAGCACTGGATGAGGGTGATCCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606130000_ixey GGAAGCAGAAGGCAGAGCTGGCCCTGGTACTG(+AAAAAA)GTACCCCACCCTTCAGCGTAGCCCTTGGTGA NGG 10 8 17 8 24 0 100 5 10
    240606125958_e8cd AGCTGTCACCAAGGGCTACGCTGAAGGGTGGG(+AAAAAA)GTACCAGTACCAGGGCCAGCTCTGCCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240606125956_ojh2 AGAGAAAAGCAGACAGGCGGGGAACCTGCCAG(+AAAAAA)GTACCAGGGCGTTCCGCAGCGAATGAGAATT NGG 10 8 17 8 24 0 100 5 10
    240606125954_gpky CCCAGAATTCTCATTCGCTGCGGAACGCCCTG(+AAAAAA)GTACCTGGCAGGTTCCCCGCCTGTCTGCTTT NGG 10 8 17 8 24 0 100 5 10