Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125952_vhsi GAGAGAAACAGAGGGATAAAGAGGAGGCTCGG(+AAAAAA)GTACCACTATGGTTCCAGAGTCCTCTGCCCT NGG 10 8 17 8 24 0 100 5 10
    240606125951_3p0q GGTAAAGGGCAGAGGACTCTGGAACCATAGTG(+AAAAAA)GTACCCGAGCCTCCTCTTTATCCCTCTGTTT NGG 10 8 17 8 24 0 100 5 10
    240606125949_cokm GGTCACAATTGTCCCTAAATCTAGTTCCAAGG(+AAAAAA)GATCCAGCATCTTCTGGCCTCCGTGGGCACC NGG 10 8 17 8 24 0 100 5 10
    240606125947_hu3q TGCCTGGTGCCCACGGAGGCCAGAAGATGCTG(+AAAAAA)GATCCCTTGGAACTAGATTTAGGGACAATTG NGG 10 8 17 8 24 0 100 5 10
    240606125946_0c2z CTTGGTTAAAATTAGTTCTCACTCCATGTATG(+AAAAAA)GATCCTTCAACTTCTCTTCAGAGAGAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240606125945_4202 TGAGGACTTCTCTCTCTGAAGAGAAGTTGAAG(+AAAAAA)GATCCATACATGGAGTGAGAACTAATTTTAA NGG 10 8 17 8 24 0 100 5 10
    240606125943_d8pz TAAGTGAGACAAGAGCCCAGCCCAACCTCAGG(+AAAAAA)GATCCAACAGTCCACGGCCATCTCCTTAGCT NGG 10 8 17 8 24 0 100 5 10
    240606125942_glgf GGGACAGCTAAGGAGATGGCCGTGGACTGTTG(+AAAAAA)GATCCCTGAGGTTGGGCTGGGCTCTTGTCTC NGG 10 8 17 8 24 0 100 5 10
    240606125941_tis1 TGTCCCTCTGACTGGATACAACCTAGTAAATG(+AAAAAA)GTACCTTCCAGCTGGGCCTGTTTCTGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240606125940_ql8t GAAGGAGCAGCAGAAACAGGCCCAGCTGGAAG(+AAAAAA)GTACCATTTACTAGGTTGTATCCAGTCAGAG NGG 10 8 17 8 24 0 100 5 10