Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606125845_ps8k GCTCACAACTGTCTATAATTCCAGTTCCAGGG(+AAAAAA)GATCCAACACCCTATTCTCCTGGTTTCCTCA NGG 10 8 17 8 24 0 100 5 10
    240606125844_cr4c ATATCTGAGGAAACCAGGAGAATAGGGTGTTG(+AAAAAA)GATCCCCTGGAACTGGAATTATAGACAGTTG NGG 10 8 17 8 24 0 100 5 10
    240606125842_7oap GTGTAGTGTTCCTAGATTCAGCTGAAGACCTG(+AAAAAA)GTACCTAGGGTTCCTAACGGGATGGTGTTAG NGG 10 8 17 8 24 0 100 5 10
    240606125840_pscs GTTTCCTAACACCATCCCGTTAGGAACCCTAG(+AAAAAA)GTACCAGGTCTTCAGCTGAATCTAGGAACAC NGG 10 8 17 8 24 0 100 5 10
    240606125838_mw48 GGCCTTGGGGACCCAGGGTCCTGGAGATGGAG(+AAAAAA)GTACCTTGGATTCTGAGACCTAATTAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240606125836_by2q GAGGGCTGACTAATTAGGTCTCAGAATCCAAG(+AAAAAA)GTACCTCCATCTCCAGGACCCTGGGTCCCCA NGG 10 8 17 8 24 0 100 5 10
    240606125835_6cuw CTTGAAAAACAGAGGCTTTGTCGTTCTCATAG(+AAAAAA)GATCCACAGTGTCTGAGCTATCCACGCTGAC NGG 10 8 17 8 24 0 100 5 10
    240606125834_hxkc TTGGTGTCAGCGTGGATAGCTCAGACACTGTG(+AAAAAA)GATCCTATGAGAACGACAAAGCCTCTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240606125831_v2ao CCCACATGCCTGGTGCATGGCCTGCTCCCTGG(+AAAAAA)GATCCCCCTGGCTGCATGCTTACTGCAGCGC NGG 10 8 17 8 24 0 100 5 10
    240606125829_qncf GCATGGCGCTGCAGTAAGCATGCAGCCAGGGG(+AAAAAA)GATCCCAGGGAGCAGGCCATGCACCAGGCAT NGG 10 8 17 8 24 0 100 5 10