Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124458_ivox GCCGCTGTTGTTTCCCAGTCAACAGTCTCCTG(+AAAAAA)GTACCACTGATGGCTGGAAGATGTCACGTTC NGG 10 8 17 8 24 0 100 5 10
    240606124457_tb9h GAGGGGAACGTGACATCTTCCAGCCATCAGTG(+AAAAAA)GTACCAGGAGACTGTTGACTGGGAAACAACA NGG 10 8 17 8 24 0 100 5 10
    240606124455_pw12 AGGCCCTGGCCTGAGGCTGATAGACCAGGCCG(+AAAAAA)GTACCCGTTTCCCTGTCCCATAGCAGAGTGA NGG 10 8 17 8 24 0 100 5 10
    240606124454_ppio TGAGGTCACTCTGCTATGGGACAGGGAAACGG(+AAAAAA)GTACCGGCCTGGTCTATCAGCCTCAGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240606124452_r42f GCTGAGCAGATAACAACTAGGACCTGCACCAG(+AAAAAA)GATCCTAAGGCAAGGGCCTGCATCTCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606124450_m19c CTGGTCCCAGGAGATGCAGGCCCTTGCCTTAG(+AAAAAA)GATCCTGGTGCAGGTCCTAGTTGTTATCTGC NGG 10 8 17 8 24 0 100 5 10
    240606124448_x1km CAGGAAGCTGGGAGCTTGGCATAGCTTATGGG(+AAAAAA)GTACCGAGTGTCCTGTGCTCTCCTCTTTGAG NGG 10 8 17 8 24 0 100 5 10
    240606124447_1fw0 TCATGCTCAAAGAGGAGAGCACAGGACACTCG(+AAAAAA)GTACCCCATAAGCTATGCCAAGCTCCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240606124446_sr4r TGTCACCAGCTGCCCTTCTCACCAGGAAACAG(+AAAAAA)GTACCTTTCCCTCTGCCTAGCGCCAACCCCA NGG 10 8 17 8 24 0 100 5 10
    240606124444_1t5w CATTGTGGGGTTGGCGCTAGGCAGAGGGAAAG(+AAAAAA)GTACCTGTTTCCTGGTGAGAAGGGCAGCTGG NGG 10 8 17 8 24 0 100 5 10