Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124443_k5op CAACCGGCCGGAGCAGCTGGGGCAGAGGATGG(+AAAAAA)GTACCACAGGTTGAGGGGATGAAGGTCTGAT NGG 10 8 17 8 24 0 100 5 10
    240606124441_31ws CCCTCATCAGACCTTCATCCCCTCAACCTGTG(+AAAAAA)GTACCCATCCTCTGCCCCAGCTGCTCCGGCC NGG 10 8 17 8 24 0 100 5 10
    240606124439_28uf AACCAGGCAGCGGCTCTCTCGATGACATTCAG(+AAAAAA)GATCCCTAAAGTCATATGCTTCCCACTATAG NGG 10 8 17 8 24 0 100 5 10
    240606124438_ia87 GGAAGCTATAGTGGGAAGCATATGACTTTAGG(+AAAAAA)GATCCTGAATGTCATCGAGAGAGCCGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240606124437_x0ch GGTAGATCTGGGAAAAGGTAGGGGAGGAATAG(+AAAAAA)GATCCAGATCAAAATACACTGTGAGTATGAA NGG 10 8 17 8 24 0 100 5 10
    240606124435_mg11 TATCATTCATACTCACAGTGTATTTTGATCTG(+AAAAAA)GATCCTATTCCTCCCCTACCTTTTCCCAGAT NGG 10 8 17 8 24 0 100 5 10
    240606124434_gcfi TTACATAAAAGGCTGACAAGGCCTTGGCTCTG(+AAAAAA)GTACCAAATGGTCCTCTGCCTGGAATGCCTT NGG 10 8 17 8 24 0 100 5 10
    240606124433_63qk GAGAGAAGGCATTCCAGGCAGAGGACCATTTG(+AAAAAA)GTACCAGAGCCAAGGCCTTGTCAGCCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240606124432_5052 GGCTCTATCTACTGAAATGAAACACACTGATG(+AAAAAA)GTACCTGGATTTCCACACCTAGTTACTTATC NGG 10 8 17 8 24 0 100 5 10
    240606124430_ye5w TTATCGATAAGTAACTAGGTGTGGAAATCCAG(+AAAAAA)GTACCATCAGTGTGTTTCATTTCAGTAGATA NGG 10 8 17 8 24 0 100 5 10