Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124346_qi3k AGGCCACTGTAAATTAAGGACTTTCTGAGAAG(+AAAAAA)GTACCCACACCTCCTTCCATGCTGGGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240606124344_p5r7 ATTAACCTGACCCAGCATGGAAGGAGGTGTGG(+AAAAAA)GTACCTTCTCAGAAAGTCCTTAATTTACAGT NGG 10 8 17 8 24 0 100 5 10
    240606124342_yrvn CCTCTGCTTCGGGTACGTACCCATTTCTGTGG(+AAAAAA)GTACCTATTAATATCTACTCTGGTATATGCT NGG 10 8 17 8 24 0 100 5 10
    240606124341_wc9f AACACAGCATATACCAGAGTAGATATTAATAG(+AAAAAA)GTACCCACAGAAATGGGTACGTACCCGAAGC NGG 10 8 17 8 24 0 100 5 10
    240606124340_wmtz AATTCTATTTTATGAGGGAGTTACTTTGTAAG(+AAAAAA)GATCCTGTCATTTTTCTGTACCCAAGGATTG NGG 10 8 17 8 24 0 100 5 10
    240606124339_4bgs AGGTTCAATCCTTGGGTACAGAAAAATGACAG(+AAAAAA)GATCCTTACAAAGTAACTCCCTCATAAAATA NGG 10 8 17 8 24 0 100 5 10
    240606124338_czkk AGACCTGCATATGGGGAGGTAACATTGTTGAG(+AAAAAA)GATCCCACTCTGGCATTCATGTTGTCGTAAA NGG 10 8 17 8 24 0 100 5 10
    240606124336_zilv CTTACTTTACGACAACATGAATGCCAGAGTGG(+AAAAAA)GATCCTCAACAATGTTACCTCCCCATATGCA NGG 10 8 17 8 24 0 100 5 10
    240606124334_yy5x CACTTGAGTAGAGATGCCCTGGGAGGCCAGGG(+AAAAAA)GATCCCAAGGCCCCATCAGAATCTTGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240606124332_itr6 AAACAAAAAACAAGATTCTGATGGGGCCTTGG(+AAAAAA)GATCCCCTGGCCTCCCAGGGCATCTCTACTC NGG 10 8 17 8 24 0 100 5 10