Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124330_q5iv TCACCTTCCCTTCTGAATCCGTTGGGGCTGGG(+AAAAAA)GTACCACCTGGATTAAGACATTTAAAATCTT NGG 10 8 17 8 24 0 100 5 10
    240606124328_7wt1 ATTCCAAGATTTTAAATGTCTTAATCCAGGTG(+AAAAAA)GTACCCCAGCCCCAACGGATTCAGAAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240606124327_eqkc TTAGGGGAAGAGACAAACTGTTCTGTACACTG(+AAAAAA)GTACCTTCTGAGTCCTGAACATTCTGGAAAC NGG 10 8 17 8 24 0 100 5 10
    240606124326_a8ad AAAATGTTTCCAGAATGTTCAGGACTCAGAAG(+AAAAAA)GTACCAGTGTACAGAACAGTTTGTCTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606124324_6bsr TTCCTTGGTATGTTCTGTTCATCACATTCTAG(+AAAAAA)GATCCCACACCAAACATCCCACGTTCTCACT NGG 10 8 17 8 24 0 100 5 10
    240606124323_rdll AGATTAGTGAGAACGTGGGATGTTTGGTGTGG(+AAAAAA)GATCCTAGAATGTGATGAACAGAACATACCA NGG 10 8 17 8 24 0 100 5 10
    240606124321_tuzq TTGTCTATAACACAGTCCACTTAAGTAAAAGG(+AAAAAA)GTACCAAATCCCCATTGCTTTGTCCTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240606124320_1lx3 AAAAAAAAGAAGGACAAAGCAATGGGGATTTG(+AAAAAA)GTACCCTTTTACTTAAGTGGACTGTGTTATA NGG 10 8 17 8 24 0 100 5 10
    240606124319_5f7c ACTACATCCATGTTAAAATCCGTGTAGTCAGG(+AAAAAA)GATCCTGTAAGTCACTCCCTATGAAGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240606124317_1taf CCCAATCCTGCTTCATAGGGAGTGACTTACAG(+AAAAAA)GATCCCTGACTACACGGATTTTAACATGGAT NGG 10 8 17 8 24 0 100 5 10