Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124302_rjzy GGGAAGTCATGGCATGTTACTGCAAAGGTCTG(+AAAAAA)GTACCCTCAACTCATAGAGTTGACAGGTTAG NGG 10 8 17 8 24 0 100 5 10
    240606124300_gfpg TGTCCCTAACCTGTCAACTCTATGAGTTGAGG(+AAAAAA)GTACCAGACCTTTGCAGTAACATGCCATGAC NGG 10 8 17 8 24 0 100 5 10
    240606124258_l2z7 GGTCAGGTTGTCACAGGAATACCCCATTCCTG(+AAAAAA)GTACCAATCTGTCTTAGTCAGGGTTTCTATT NGG 10 8 17 8 24 0 100 5 10
    240606124257_gnxc GCAGGAATAGAAACCCTGACTAAGACAGATTG(+AAAAAA)GTACCAGGAATGGGGTATTCCTGTGACAACC NGG 10 8 17 8 24 0 100 5 10
    240606124256_sqc3 ATGGAACCAGCGTCTGTGAAAAGTCAGGATTG(+AAAAAA)GTACCAACACGTGTGAGTGAAAACTCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240606124255_qwve AGAAATCCCAGAGTTTTCACTCACACGTGTTG(+AAAAAA)GTACCAATCCTGACTTTTCACAGACGCTGGT NGG 10 8 17 8 24 0 100 5 10
    240606124254_ac5q CAGCCTGGGAAGGTGGGAGGCAGAGAACACAG(+AAAAAA)GTACCTCCCTGGAAGTACATGAGCTATCTGG NGG 10 8 17 8 24 0 100 5 10
    240606124252_wmxu CTTTGCCAGATAGCTCATGTACTTCCAGGGAG(+AAAAAA)GTACCTGTGTTCTCTGCCTCCCACCTTCCCA NGG 10 8 17 8 24 0 100 5 10
    240606124251_oot4 ATGATGGTGCTTGGTGAGTCGTGGTTCTAAAG(+AAAAAA)GTACCAAACGGGGGAGCCAAAGTTCTCCAAC NGG 10 8 17 8 24 0 100 5 10
    240606124249_hyct CAGCAGTTGGAGAACTTTGGCTCCCCCGTTTG(+AAAAAA)GTACCTTTAGAACCACGACTCACCAAGCACC NGG 10 8 17 8 24 0 100 5 10