Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124248_zfjq AATAACTTTAATTTACATACTCACTCACACAG(+AAAAAA)GTACCAGTGCTTTGAAAATAGATTGTTCAGT NGG 10 8 17 8 24 0 100 5 10
    240606124247_l9k9 TTAGGACTGAACAATCTATTTTCAAAGCACTG(+AAAAAA)GTACCTGTGTGAGTGAGTATGTAAATTAAAG NGG 10 8 17 8 24 0 100 5 10
    240606124246_7clc TTGTGTTTAAACCCTTGGCTTCAAGGAGTCAG(+AAAAAA)GATCCCCCTATCTTTCATCAAGTTTCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606124243_x755 AGTGTTTCCTGAAACTTGATGAAAGATAGGGG(+AAAAAA)GATCCTGACTCCTTGAAGCCAAGGGTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240606124242_s3j8 TCTCGGATACAATCGATGCGTCTGACCTTTGG(+AAAAAA)GATCCTCACCATATGGGCAAACAATGGGCTT NGG 10 8 17 8 24 0 100 5 10
    240606124241_p2rh CTGCAAAGCCCATTGTTTGCCCATATGGTGAG(+AAAAAA)GATCCCAAAGGTCAGACGCATCGATTGTATC NGG 10 8 17 8 24 0 100 5 10
    240606124240_mga4 AATAATGTTAACATTATTATATAAACAACTTG(+AAAAAA)GATCCCAAATTCTTGGAAGTTCATGTGTAAA NGG 10 8 17 8 24 0 100 5 10
    240606124238_bax0 ACGTTTTTACACATGAACTTCCAAGAATTTGG(+AAAAAA)GATCCAAGTTGTTTATATAATAATGTTAACA NGG 10 8 17 8 24 0 100 5 10
    240606124237_s4ds TTTAGACCTCCATAGCTACAAATTTAAAATAG(+AAAAAA)GTACCTTCCACATTGTCCAATGAATCCTATG NGG 10 8 17 8 24 0 100 5 10
    240606124235_mt5b GACTCCATAGGATTCATTGGACAATGTGGAAG(+AAAAAA)GTACCTATTTTAAATTTGTAGCTATGGAGGT NGG 10 8 17 8 24 0 100 5 10