Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124218_g4t8 CCACTTTATCATAGAAATACCCCACTCAGCTG(+AAAAAA)GTACCAATTTGCCTTAGTCATGGTTTCTATT NGG 10 8 17 8 24 0 100 5 10
    240606124217_trvd CCAGGAATAGAAACCATGACTAAGGCAAATTG(+AAAAAA)GTACCAGCTGAGTGGGGTATTTCTATGATAA NGG 10 8 17 8 24 0 100 5 10
    240606124215_g4cz CTCACCTCCCAGGTCTCTGGTACATTCTGGAG(+AAAAAA)GATCCCTGCACCTCTTTCTTCCCAAGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240606124213_e69v AACAAACAACCTTGGGAAGAAAGAGGTGCAGG(+AAAAAA)GATCCTCCAGAATGTACCAGAGACCTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240606124212_0ldh CCACATTGTCACAGGAATGCCCTACTATGCTG(+AAAAAA)GTACCAATTTGTCTTAGTCAGGGTATCTATT NGG 10 8 17 8 24 0 100 5 10
    240606124211_t2bl CCAGGAATAGATACCCTGACTAAGACAAATTG(+AAAAAA)GTACCAGCATAGTAGGGCATTCCTGTGACAA NGG 10 8 17 8 24 0 100 5 10
    240606124209_rl36 AAACATTCCTAAATCATGGACCCACTGGGCAG(+AAAAAA)GATCCCTATGGGGGAGGATTCCATACTTGAG NGG 10 8 17 8 24 0 100 5 10
    240606124208_9rmd TAAAACTCAAGTATGGAATCCTCCCCCATAGG(+AAAAAA)GATCCTGCCCAGTGGGTCCATGATTTAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606124206_9jrz GATAAAAACTAAGAACAGAAACCCCACGAAAG(+AAAAAA)GATCCTACTTCTCGTCTCTCATTGCAGGATA NGG 10 8 17 8 24 0 100 5 10
    240606124205_pr28 AGATATATCCTGCAATGAGAGACGAGAAGTAG(+AAAAAA)GATCCTTTCGTGGGGTTTCTGTTCTTAGTTT NGG 10 8 17 8 24 0 100 5 10