Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124117_zaqg GGAGGTTCAGTGACCAGTAGGCCTCCCCACTG(+AAAAAA)GTACCACCACTTCCACTGTGAGACTGCAGAA NGG 10 8 17 8 24 0 100 5 10
    240606124115_9f5j TTGAGTTCTGCAGTCTCACAGTGGAAGTGGTG(+AAAAAA)GTACCAGTGGGGAGGCCTACTGGTCACTGAA NGG 10 8 17 8 24 0 100 5 10
    240606124114_dt8b AAGATTCTAAAGAGCGTTGAAGTGTTTAAAAG(+AAAAAA)GATCCACGAGGACAAGTTAGGTTGATGGTAT NGG 10 8 17 8 24 0 100 5 10
    240606124113_lkv8 GATACATACCATCAACCTAACTTGTCCTCGTG(+AAAAAA)GATCCTTTTAAACACTTCAACGCTCTTTAGA NGG 10 8 17 8 24 0 100 5 10
    240606124111_dcz3 GTGAGTGACTTGGGGGTGGGTTTGAGTCTGGG(+AAAAAA)GTACCCAGTGACACAATTTTCTCATCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240606124108_se94 TAGAGTTCCAGATGAGAAAATTGTGTCACTGG(+AAAAAA)GTACCCCAGACTCAAACCCACCCCCAAGTCA NGG 10 8 17 8 24 0 100 5 10
    240606124105_xwcs GAAACGTCCTTCCTCCCCTTCGCCCTGCCTGG(+AAAAAA)GATCCAGGTAGGGTGGTTGGACCTCTCCATC NGG 10 8 17 8 24 0 100 5 10
    240606124104_djvn ACAAGGATGGAGAGGTCCAACCACCCTACCTG(+AAAAAA)GATCCCAGGCAGGGCGAAGGGGAGGAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240606124102_vf5r GAATGCCCTGGCATCTTGGTGGCATCTTGATG(+AAAAAA)GATCCAGCTCAGTCAGGACAGCTTTGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240606124101_g5or GCTCTGTGCTCAAAGCTGTCCTGACTGAGCTG(+AAAAAA)GATCCATCAAGATGCCACCAAGATGCCAGGG NGG 10 8 17 8 24 0 100 5 10