Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124045_vnfk TCTGTGTGAGTGTACACCACTTGTGTGGAGAG(+AAAAAA)GTACCCATGGAGCCCAGAGGTAGTGTATCCC NGG 10 8 17 8 24 0 100 5 10
    240606124042_zlb3 TCCAGGGGATACACTACCTCTGGGCTCCATGG(+AAAAAA)GTACCTCTCCACACAAGTGGTGTACACTCAC NGG 10 8 17 8 24 0 100 5 10
    240606124041_1xiq TGCACACACCCATCTTCGCTACTCCAGACTAG(+AAAAAA)GATCCATAGCAATTGGGAAATGGGCATTGGG NGG 10 8 17 8 24 0 100 5 10
    240606124040_3ibp TACTCCCCAATGCCCATTTCCCAATTGCTATG(+AAAAAA)GATCCTAGTCTGGAGTAGCGAAGATGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240606124038_5ojd CCCAGGAAATGCCTCCCTCCGGTCCAGGCAGG(+AAAAAA)GATCCCAGCACACACACCCCTGCTGGTGGTG NGG 10 8 17 8 24 0 100 5 10
    240606124037_asu6 CCCTCCACCACCAGCAGGGGTGTGTGTGCTGG(+AAAAAA)GATCCCTGCCTGGACCGGAGGGAGGCATTTC NGG 10 8 17 8 24 0 100 5 10
    240606124035_umfq GTCCCACTTTCAATGGGAGCTATGAGGTATGG(+AAAAAA)GTACCAGGCTTAGCAAGCAAGTTGTGGTCCC NGG 10 8 17 8 24 0 100 5 10
    240606124033_4vu1 CTGTGGGGACCACAACTTGCTTGCTAAGCCTG(+AAAAAA)GTACCCATACCTCATAGCTCCCATTGAAAGT NGG 10 8 17 8 24 0 100 5 10
    240606124032_1qbf TTGTTCAGGGACTACTCAAGAAAATATCTTTG(+AAAAAA)GATCCTTAGTTGGCCTTCAAGTCTATACATA NGG 10 8 17 8 24 0 100 5 10
    240606124031_gxul TCTTATATGTATAGACTTGAAGGCCAACTAAG(+AAAAAA)GATCCAAAGATATTTTCTTGAGTAGTCCCTG NGG 10 8 17 8 24 0 100 5 10