Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606124010_k7w8 GTTGAGGAGAGCAGCATATTCATGGAGCGCTG(+AAAAAA)GATCCTCTGGGGCAGCCCCCAGCGGAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240606124009_9n4f CTGATTCACCTCCGCTGGGGGCTGCCCCAGAG(+AAAAAA)GATCCAGCGCTCCATGAATATGCTGCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240606124007_ek37 CTAGACTGCAGTGCTTCCAATAAAGCTGCTTG(+AAAAAA)GTACCGTGGATCTGTCTGTCGGTTGTCTGTT NGG 10 8 17 8 24 0 100 5 10
    240606124006_iysa GAGAGAACAGACAACCGACAGACAGATCCACG(+AAAAAA)GTACCAAGCAGCTTTATTGGAAGCACTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606124004_6rk3 GTCATGTAACCCCTATGTCAGTGTCACCTTTG(+AAAAAA)GTACCCCACCTCTTTGAGTCAATTCTGCTCC NGG 10 8 17 8 24 0 100 5 10
    240606124001_c4gs GAAAAGGAGCAGAATTGACTCAAAGAGGTGGG(+AAAAAA)GTACCAAAGGTGACACTGACATAGGGGTTAC NGG 10 8 17 8 24 0 100 5 10
    240606124000_pm1s TTGATTCTTTCAGGATGTGACGCCCACTCTGG(+AAAAAA)GATCCAGTCCTGGTTGATGTCAGCATGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606123958_fr7z TGGAGGTGGCATGCTGACATCAACCAGGACTG(+AAAAAA)GATCCCAGAGTGGGCGTCACATCCTGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606123957_ks61 CCTGAGTATACCTGAGTCAGAGCGTGGAATCG(+AAAAAA)GATCCAAGAAGTCAGTCGGCCTGGGGTCCAG NGG 10 8 17 8 24 0 100 5 10
    240606123956_574y ATCGACTGGACCCCAGGCCGACTGACTTCTTG(+AAAAAA)GATCCGATTCCACGCTCTGACTCAGGTATAC NGG 10 8 17 8 24 0 100 5 10