Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123923_6zcx GCTTTGAACTTGCAGAGATCTGCCAGTCTGTG(+AAAAAA)GATCCAGGCTGCTGGGATGCAAGTACACCAC NGG 10 8 17 8 24 0 100 5 10
    240606123921_6jwc TTTTTGTGGTGTACTTGCATCCCAGCAGCCTG(+AAAAAA)GATCCACAGACTGGCAGATCTCTGCAAGTTC NGG 10 8 17 8 24 0 100 5 10
    240606123920_cq9e ATCTACAAGGACTTGTTCACTTCAAAGGAAGG(+AAAAAA)GATCCCATCGCTGCTATGAATCTGAAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606123918_cidl TTCTTCACACTTCAGATTCATAGCAGCGATGG(+AAAAAA)GATCCCTTCCTTTGAAGTGAACAAGTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240606123916_76x9 TGGTTTCTCCAGAAGGCGGTACATCGCTAGGG(+AAAAAA)GTACCTATAGAATACAAGGTAGGTGTCACTC NGG 10 8 17 8 24 0 100 5 10
    240606123915_lcn7 TTTAAGAGTGACACCTACCTTGTATTCTATAG(+AAAAAA)GTACCCCTAGCGATGTACCGCCTTCTGGAGA NGG 10 8 17 8 24 0 100 5 10
    240606123913_yase GCCATTTCTGTACACACTGTTCTCTTGTTCAG(+AAAAAA)GTACCTGGAGAGGAAGGCTAGCCTTCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240606123911_yb4l GACTGGAGAAGAAGGCTAGCCTTCCTCTCCAG(+AAAAAA)GTACCTGAACAAGAGAACAGTGTGTACAGAA NGG 10 8 17 8 24 0 100 5 10
    240606123910_pm86 GTCTGCGAGTGTGGCCGCAAGGAAAATGGGAG(+AAAAAA)GATCCAACAGTCCTCTTTATTCTTTTGCTTC NGG 10 8 17 8 24 0 100 5 10
    240606123909_9cp1 GCCAGGAAGCAAAAGAATAAAGAGGACTGTTG(+AAAAAA)GATCCTCCCATTTTCCTTGCGGCCACACTCG NGG 10 8 17 8 24 0 100 5 10