Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123837_3zz2 CATTGCTGTGTTCGAATAAAGCAAGCCACAGG(+AAAAAA)GTACCGTGAGGGAAAGCGTGAGGCACGCCGC NGG 10 8 17 8 24 0 100 5 10
    240606123836_6k00 GTTCTGCGGCGTGCCTCACGCTTTCCCTCACG(+AAAAAA)GTACCCTGTGGCTTGCTTTATTCGAACACAG NGG 10 8 17 8 24 0 100 5 10
    240606123833_47zq CACCAAGCCTGATAGCCTGCATTCGATCCCGG(+AAAAAA)GATCCATGTGGTACAAGAAGAGAGTTAGAAT NGG 10 8 17 8 24 0 100 5 10
    240606123832_0ric AGTCAATTCTAACTCTCTTCTTGTACCACATG(+AAAAAA)GATCCCGGGATCGAATGCAGGCTATCAGGCT NGG 10 8 17 8 24 0 100 5 10
    240606123830_l5vb GTTGACACGTGCTGCTGTGTTTATTTAATATG(+AAAAAA)GATCCACATCAGGAAAGAGGGAATAATACAC NGG 10 8 17 8 24 0 100 5 10
    240606123829_wmzg AAAAAGTGTATTATTCCCTCTTTCCTGATGTG(+AAAAAA)GATCCATATTAAATAAACACAGCAGCACGTG NGG 10 8 17 8 24 0 100 5 10
    240606123828_2704 TGGCAATGCCTGTTCATCACCCCTCTCTAAGG(+AAAAAA)GTACCGTGGTCAAATAAGTTGCGCTACAACT NGG 10 8 17 8 24 0 100 5 10
    240606123826_pu3m TGTGAAGTTGTAGCGCAACTTATTTGACCACG(+AAAAAA)GTACCCTTAGAGAGGGGTGATGAACAGGCAT NGG 10 8 17 8 24 0 100 5 10
    240606123824_cfdl GGTTTTCTAATGAAAACAAAAATGGATTTCAG(+AAAAAA)GTACCATGCTGATATTTGTTTTGTCTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240606123823_s0mu CGTCTAAAACAGACAAAACAAATATCAGCATG(+AAAAAA)GTACCTGAAATCCATTTTTGTTTTCATTAGA NGG 10 8 17 8 24 0 100 5 10