Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123907_rsb8 GGTTAGGAAATGATATGGTCAAAGCCAAAGTG(+AAAAAA)GATCCAACTTATTTTAAGTCCTTTTAAATGA NGG 10 8 17 8 24 0 100 5 10
    240606123906_5153 TACCTTCATTTAAAAGGACTTAAAATAAGTTG(+AAAAAA)GATCCACTTTGGCTTTGACCATATCATTTCC NGG 10 8 17 8 24 0 100 5 10
    240606123905_fa3z ACTAGGCTGCCTGGAGTCCACAGGCAGTTCTG(+AAAAAA)GATCCACCATGTAAACGACAGAAGGAACCTT NGG 10 8 17 8 24 0 100 5 10
    240606123903_zl8n TTGTCAAGGTTCCTTCTGTCGTTTACATGGTG(+AAAAAA)GATCCAGAACTGCCTGTGGACTCCAGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240606123902_d29p TAAGGCACATGGAAGACTGTTTATTTGCACAG(+AAAAAA)GTACCGAAATTTTTTTAAGAACAGCTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240606123901_vq7h GAGATTTGACAGCTGTTCTTAAAAAAATTTCG(+AAAAAA)GTACCTGTGCAAATAAACAGTCTTCCATGTG NGG 10 8 17 8 24 0 100 5 10
    240606123859_3ctl ATATCTGGGTTGGGACCTAGGATTTGTCCTCG(+AAAAAA)GATCCTTTGGAACACAGAACATGGATTATGT NGG 10 8 17 8 24 0 100 5 10
    240606123857_2gsl GACGGACATAATCCATGTTCTGTGTTCCAAAG(+AAAAAA)GATCCGAGGACAAATCCTAGGTCCCAACCCA NGG 10 8 17 8 24 0 100 5 10
    240606123856_fjdm GTCTAGCACTTACAGCATTAGCCTCCTTGATG(+AAAAAA)GATCCACTACTACCTGCATTTTGACTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240606123855_kgzf CTCTTTGAGAAGTCAAAATGCAGGTAGTAGTG(+AAAAAA)GATCCATCAAGGAGGCTAATGCTGTAAGTGC NGG 10 8 17 8 24 0 100 5 10