Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123808_bocl GGAACAAAGCACCATTGGCCTCTAGCTCTGGG(+AAAAAA)GATCCTACAGCGTCACCTTCCTCCTAGACAG NGG 10 8 17 8 24 0 100 5 10
    240606123807_ijy9 TCCTGCTGTCTAGGAGGAAGGTGACGCTGTAG(+AAAAAA)GATCCCCAGAGCTAGAGGCCAATGGTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240606123806_pnwy AAGGTTTGCCCTTTAGTTTAGAAACCAGATAG(+AAAAAA)GATCCCATCCTTGAATTTTAAATGAAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240606123804_srwu TGCAAATGACTTCATTTAAAATTCAAGGATGG(+AAAAAA)GATCCTATCTGGTTTCTAAACTAAAGGGCAA NGG 10 8 17 8 24 0 100 5 10
    240606123803_xfce GCAGTGCTGGTAGAATCCAGTAGAGGATATTG(+AAAAAA)GATCCTCTGTGACTAGAATTACAGATAGTTA NGG 10 8 17 8 24 0 100 5 10
    240606123801_an8p GTTCATAACTATCTGTAATTCTAGTCACAGAG(+AAAAAA)GATCCAATATCCTCTACTGGATTCTACCAGC NGG 10 8 17 8 24 0 100 5 10
    240606123800_021n CCAGACAAATGTAGTTTACTGCAGGCTTACTG(+AAAAAA)GATCCTGACATTTAATTAAATATAACATAAT NGG 10 8 17 8 24 0 100 5 10
    240606123759_x9ht ACTAGATTATGTTATATTTAATTAAATGTCAG(+AAAAAA)GATCCAGTAAGCCTGCAGTAAACTACATTTG NGG 10 8 17 8 24 0 100 5 10
    240606123757_vpk4 ATAACATGGCAATAATGAAGTGCTGTTTCCTG(+AAAAAA)GTACCTTGGGAATACTAAAGAAAGAAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240606123754_xq1m GCAATGCTTTTTCTTTCTTTAGTATTCCCAAG(+AAAAAA)GTACCAGGAAACAGCACTTCATTATTGCCAT NGG 10 8 17 8 24 0 100 5 10