Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123739_kx7b AAAAGGATATACCTTCTTCTCAGCACCTCATG(+AAAAAA)GTACCTTCTCCAAAATTGACCACATAATAGG NGG 10 8 17 8 24 0 100 5 10
    240606123738_i04x TGTGACCTATTATGTGGTCAATTTTGGAGAAG(+AAAAAA)GTACCATGAGGTGCTGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10
    240606123736_n5ub ACGAAATGGACAAATTTCTGGACAGATACCAG(+AAAAAA)GTACCAAAGTTGAATCAGGATCAAGTTGACC NGG 10 8 17 8 24 0 100 5 10
    240606123734_zjd7 TAGAAGGTCAACTTGATCCTGATTCAACTTTG(+AAAAAA)GTACCTGGTATCTGTCCAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240606123733_ga98 ATCTGTGTAAAGTTTGCATTAGTTAGAAGATG(+AAAAAA)GTACCGAGTTTAATTGACATAATAAAAGCTT NGG 10 8 17 8 24 0 100 5 10
    240606123732_byis TGGGCAAGCTTTTATTATGTCAATTAAACTCG(+AAAAAA)GTACCATCTTCTAACTAATGCAAACTTTACA NGG 10 8 17 8 24 0 100 5 10
    240606123730_3go7 GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+AAAAAA)GATCCCTGGATAAGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606123728_t30v GGATGGACCATGTAGAGACTGCCTTATCCAGG(+AAAAAA)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240606123727_4cqr TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+AAAAAA)GATCCTCCGGTAGTACTATGTCCAATTTACT NGG 10 8 17 8 24 0 100 5 10
    240606123725_srkq TCCTCAGTAAATTGGACATAGTACTACCGGAG(+AAAAAA)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10