Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123634_sgkq ATTTCTTCAAAGAGAGACAAGGTTCATGCCTG(+AAAAAA)GTACCATTTTTTTTCTAATGAAATTAACTTT NGG 10 8 17 8 24 0 100 5 10
    240606123633_a0q7 CTAAAAAAGTTAATTTCATTAGAAAAAAAATG(+AAAAAA)GTACCAGGCATGAACCTTGTCTCTCTTTGAA NGG 10 8 17 8 24 0 100 5 10
    240606123631_r2iy TACCTTTAATCTTAGCACTTGAGAGGCAGAGG(+AAAAAA)GATCCCCGAGTTAGAGATCAGCCTTGTTTAT NGG 10 8 17 8 24 0 100 5 10
    240606123629_f76d TTGAAATAAACAAGGCTGATCTCTAACTCGGG(+AAAAAA)GATCCCTCTGCCTCTCAAGTGCTAAGATTAA NGG 10 8 17 8 24 0 100 5 10
    240606123627_53rf ATTTCTTCAAAGAGAGACAAGGTTCATGCCTG(+AAAAAA)GTACCATTTTTTTTCTAATGAAATTAACTTT NGG 10 8 17 8 24 0 100 5 10
    240606123626_71y2 CTAAAAAAGTTAATTTCATTAGAAAAAAAATG(+AAAAAA)GTACCAGGCATGAACCTTGTCTCTCTTTGAA NGG 10 8 17 8 24 0 100 5 10
    240606123625_399v GTGGTGCACTCCAACAGCATTTTAAGTACTTG(+AAAAAA)GATCCCACGTTGATCTTATAAACACAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240606123623_80qi TTTTATTGCTTGTGTTTATAAGATCAACGTGG(+AAAAAA)GATCCAAGTACTTAAAATGCTGTTGGAGTGC NGG 10 8 17 8 24 0 100 5 10
    240606123621_wxaw TCTCTGTCTGACTTCCTATGCATGGGGGCTGG(+AAAAAA)GATCCATTTCTAAGGAGAATGGATAAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606123620_2dhs GCCTACTTCCTTATCCATTCTCCTTAGAAATG(+AAAAAA)GATCCCAGCCCCCATGCATAGGAAGTCAGAC NGG 10 8 17 8 24 0 100 5 10