Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123535_79pa TTGAGTTGAACTAGTGTTGAGGCTAGGCTTAG(+AAAAAA)GTACCATAGAACTTTGGTTTTTCAAGACAGG NGG 10 8 17 8 24 0 100 5 10
    240606123533_9w0b GAAACCCTGTCTTGAAAAACCAAAGTTCTATG(+AAAAAA)GTACCTAAGCCTAGCCTCAACACTAGTTCAA NGG 10 8 17 8 24 0 100 5 10
    240606123532_pwtr CTGACTGGCTCTGGCCTATGAACTCATTGCTG(+AAAAAA)GATCCATTAACCCTTTCCTAGCTTCAGAGGT NGG 10 8 17 8 24 0 100 5 10
    240606123531_qqbo GAATTACCTCTGAAGCTAGGAAAGGGTTAATG(+AAAAAA)GATCCAGCAATGAGTTCATAGGCCAGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240606123529_co1p ACTTTCTCTGAGCTGCTTTTATGCATATTGGG(+AAAAAA)GTACCTAGTAGTCTTCCCAACCTTTAACACT NGG 10 8 17 8 24 0 100 5 10
    240606123528_wfuo CTCACAGTGTTAAAGGTTGGGAAGACTACTAG(+AAAAAA)GTACCCCAATATGCATAAAAGCAGCTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240606123527_gb15 AAAGAACATGAAAAATTCAGAGGAGAAATGAG(+AAAAAA)GTACCCATAGTCAACAGAGCGTTTTACTGAG NGG 10 8 17 8 24 0 100 5 10
    240606123525_fypt TGTATCTCAGTAAAACGCTCTGTTGACTATGG(+AAAAAA)GTACCTCATTTCTCCTCTGAATTTTTCATGT NGG 10 8 17 8 24 0 100 5 10
    240606123523_qey1 TGGTACTAGGGTTTCCTGTAAACACCTTGTGG(+AAAAAA)GTACCTCATAAGCAGCTGAGATTATTAGTGT NGG 10 8 17 8 24 0 100 5 10
    240606123522_0y7w ACCAAACACTAATAATCTCAGCTGCTTATGAG(+AAAAAA)GTACCCACAAGGTGTTTACAGGAAACCCTAG NGG 10 8 17 8 24 0 100 5 10