Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123434_nvht AATACTGACTCTCATTAGATATGATCTTATAG(+AAAAAA)GATCCATACCCTCCCTAGGACCAAATATATC NGG 10 8 17 8 24 0 100 5 10
    240606123432_amxk CATCAGATATATTTGGTCCTAGGGAGGGTATG(+AAAAAA)GATCCTATAAGATCATATCTAATGAGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240606123430_xe2n GGCATGGCTCCTCGCCGCAGCTGTCTCCCTTG(+AAAAAA)GTACCGACCTCTCTGTGGGCTGGAGAGAGGC NGG 10 8 17 8 24 0 100 5 10
    240606123428_jrq3 CTTAGGCCTCTCTCCAGCCCACAGAGAGGTCG(+AAAAAA)GTACCAAGGGAGACAGCTGCGGCGAGGAGCC NGG 10 8 17 8 24 0 100 5 10
    240606123427_0xpr CCTTGTCAAACCAAGTTGTCATGGATGCTTTG(+AAAAAA)GTACCCCTCCCCTCAGGGGCTTTGGTATTAC NGG 10 8 17 8 24 0 100 5 10
    240606123425_e78i TTCTTGTAATACCAAAGCCCCTGAGGGGAGGG(+AAAAAA)GTACCAAAGCATCCATGACAACTTGGTTTGA NGG 10 8 17 8 24 0 100 5 10
    240606123424_wdqt GTGGGGTTCCTGCGCTGTGTGTGCCTACTCAG(+AAAAAA)GTACCCCAACATTGCTTTCCCTAGCTCTCTG NGG 10 8 17 8 24 0 100 5 10
    240606123422_tr3l CACAGCAGAGAGCTAGGGAAAGCAATGTTGGG(+AAAAAA)GTACCTGAGTAGGCACACACAGCGCAGGAAC NGG 10 8 17 8 24 0 100 5 10
    240606123421_keam GCTCAATGCCATCATCACTGTGATTAAGTGAG(+AAAAAA)GTACCAGGGTTCATAGCGTCGGAGACTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606123418_x2o7 AGCGTCAGCAGTCTCCGACGCTATGAACCCTG(+AAAAAA)GTACCTCACTTAATCACAGTGATGATGGCAT NGG 10 8 17 8 24 0 100 5 10