Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123448_st54 TCCTGTGGTTTTGTAAACAGAGATATTGGGGG(+AAAAAA)GATCCTCCTTGAAATAGTTTGGAAGAATTGC NGG 10 8 17 8 24 0 100 5 10
    240606123447_p15x ACAGTGCAATTCTTCCAAACTATTTCAAGGAG(+AAAAAA)GATCCCCCCAATATCTCTGTTTACAAAACCA NGG 10 8 17 8 24 0 100 5 10
    240606123444_7vbe AGAACTATACATAGTGAGACCATGTAACCCTG(+AAAAAA)GATCCTAACAACACAGTCTGTAAATAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240606123443_u5nh AATAAACTTTTATTTACAGACTGTGTTGTTAG(+AAAAAA)GATCCAGGGTTACATGGTCTCACTATGTATA NGG 10 8 17 8 24 0 100 5 10
    240606123441_osg1 TAGGTGACATTCAGAGATGCTGTGTTGGGCTG(+AAAAAA)GTACCAGAATGCATTGATGTGTGTGCTTTAC NGG 10 8 17 8 24 0 100 5 10
    240606123440_98ps CAACGGTAAAGCACACACATCAATGCATTCTG(+AAAAAA)GTACCAGCCCAACACAGCATCTCTGAATGTC NGG 10 8 17 8 24 0 100 5 10
    240606123439_0rp3 TATGCTACTTGCACTGTGACATGGCATTTGAG(+AAAAAA)GATCCCTGACTTACAGACAAAGCTTAAAATG NGG 10 8 17 8 24 0 100 5 10
    240606123437_tkuz TGTGTCATTTTAAGCTTTGTCTGTAAGTCAGG(+AAAAAA)GATCCTCAAATGCCATGTCACAGTGCAAGTA NGG 10 8 17 8 24 0 100 5 10
    240606123436_apb0 AAAGCTCATCACAAGGTCTACTAAGCATTAAG(+AAAAAA)GATCCTCATTATAAGTCCTCTGTACCACTTT NGG 10 8 17 8 24 0 100 5 10
    240606123435_07eo AAAAAAAAGTGGTACAGAGGACTTATAATGAG(+AAAAAA)GATCCTTAATGCTTAGTAGACCTTGTGATGA NGG 10 8 17 8 24 0 100 5 10