Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123417_qy9w ACAGGAACCAGTCCCTAAAAGTGCTCTTCTCG(+AAAAAA)GTACCTGGCGAGGCAATCATTCCAGCGCTTC NGG 10 8 17 8 24 0 100 5 10
    240606123415_qcxs AAACAGAAGCGCTGGAATGATTGCCTCGCCAG(+AAAAAA)GTACCGAGAAGAGCACTTTTAGGGACTGGTT NGG 10 8 17 8 24 0 100 5 10
    240606123414_48y8 AGAATCTAGTAGCCACACAATGGGGGGCTCAG(+AAAAAA)GTACCATTATTTACTACTTTCTTTTGACCTA NGG 10 8 17 8 24 0 100 5 10
    240606123413_5a96 AGTCCTAGGTCAAAAGAAAGTAGTAAATAATG(+AAAAAA)GTACCTGAGCCCCCCATTGTGTGGCTACTAG NGG 10 8 17 8 24 0 100 5 10
    240606123411_zy8h AAATACTCGATGTGCAATTACTTTGCACTTGG(+AAAAAA)GATCCAGCAAGCCCCTCAGCGGTATCCTGAT NGG 10 8 17 8 24 0 100 5 10
    240606123410_jqra TGGTTATCAGGATACCGCTGAGGGGCTTGCTG(+AAAAAA)GATCCCAAGTGCAAAGTAATTGCACATCGAG NGG 10 8 17 8 24 0 100 5 10
    240606123409_4wz6 AATGACATGAACTTATTAAGAATTAACTTAAG(+AAAAAA)GATCCAATATATATATATATATATAATATGT NGG 10 8 17 8 24 0 100 5 10
    240606123407_v4rs ATATAACATATTATATATATATATATATATTG(+AAAAAA)GATCCTTAAGTTAATTCTTAATAAGTTCATG NGG 10 8 17 8 24 0 100 5 10
    240606123406_1cjn TGCTTAATTCTTTTAGCGAATGCACAGGTTAG(+AAAAAA)GTACCACCGTGAGGAAAAGGAGGGTGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606123405_9tmw TCATTCCCCACACCCTCCTTTTCCTCACGGTG(+AAAAAA)GTACCTAACCTGTGCATTCGCTAAAAGAATT NGG 10 8 17 8 24 0 100 5 10