Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123349_apkc ATGTTCCAGTACCAATGGAAGAACTGCTATAG(+AAAAAA)GTACCTGCCTAATAAGATTTCTAAACCCATA NGG 10 8 17 8 24 0 100 5 10
    240606123347_upvs TTAACTATGGGTTTAGAAATCTTATTAGGCAG(+AAAAAA)GTACCTATAGCAGTTCTTCCATTGGTACTGG NGG 10 8 17 8 24 0 100 5 10
    240606123345_p97j TTTGGCTTTCACTGGATGTGCCACGGGGTGGG(+AAAAAA)GATCCTGTCTGATTCTGAATCAATTGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240606123344_qd1c TGCATCTGGCCAATTGATTCAGAATCAGACAG(+AAAAAA)GATCCCCACCCCGTGGCACATCCAGTGAAAG NGG 10 8 17 8 24 0 100 5 10
    240606123342_9yyk AATGTTTATTCCACCTTTCTGTCATATTCCTG(+AAAAAA)GATCCTTCACCAATGTTACACGAGGAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606123341_5myg TTTGTTTTTTTCCTCGTGTAACATTGGTGAAG(+AAAAAA)GATCCAGGAATATGACAGAAAGGTGGAATAA NGG 10 8 17 8 24 0 100 5 10
    240606123340_nju7 CTGCTTTGACAGGATATCTGCTCTCAGAAACG(+AAAAAA)GATCCATAACTCGTTAGTGAGTGACTCCTAG NGG 10 8 17 8 24 0 100 5 10
    240606123339_wq1k CCTAACTAGGAGTCACTCACTAACGAGTTATG(+AAAAAA)GATCCGTTTCTGAGAGCAGATATCCTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240606123337_071y TTTCTAGACACAGTTGACTGTCAATGAACTGG(+AAAAAA)GATCCTACAAGATGACAAGATGTTCAACTCA NGG 10 8 17 8 24 0 100 5 10
    240606123336_cyui GTGTGTGAGTTGAACATCTTGTCATCTTGTAG(+AAAAAA)GATCCCAGTTCATTGACAGTCAACTGTGTCT NGG 10 8 17 8 24 0 100 5 10