Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123249_swnb CTTTCTATTGAGTTGTCCCTGCCACCCATAAG(+AAAAAA)GTACCAGGTGAGCCATTTCTTTTCATCTGTA NGG 10 8 17 8 24 0 100 5 10
    240606123247_0037 GCGCATACAGATGAAAAGAAATGGCTCACCTG(+AAAAAA)GTACCTTATGGGTGGCAGGGACAACTCAATA NGG 10 8 17 8 24 0 100 5 10
    240606123245_1wyk AGTAGGTGACCCCATAGGTGTACCACCATAGG(+AAAAAA)GTACCTGCACCCACACCTGTGTACTCACAAA NGG 10 8 17 8 24 0 100 5 10
    240606123244_3s8z TTTAATTTGTGAGTACACAGGTGTGGGTGCAG(+AAAAAA)GTACCCTATGGTGGTACACCTATGGGGTCAC NGG 10 8 17 8 24 0 100 5 10
    240606123243_3h07 AGATAGTTTTCAGTCTTTGACTCTGATGAATG(+AAAAAA)GTACCACCGTGAGCATTCTTGTATATGCATG NGG 10 8 17 8 24 0 100 5 10
    240606123241_nyas AGAAACATGCATATACAAGAATGCTCACGGTG(+AAAAAA)GTACCATTCATCAGAGTCAAAGACTGAAAAC NGG 10 8 17 8 24 0 100 5 10
    240606123240_x1gr TTTAATATCGTGTGTCTCGGGAATGCAAGATG(+AAAAAA)GATCCCAAAGGTTTTACTGTTTCCCTGCCTT NGG 10 8 17 8 24 0 100 5 10
    240606123238_ayrf GCTAAAAGGCAGGGAAACAGTAAAACCTTTGG(+AAAAAA)GATCCATCTTGCATTCCCGAGACACACGATA NGG 10 8 17 8 24 0 100 5 10
    240606123237_3xie TGGGACAAGGATGAGCAAGCCCTGGGCAGGTG(+AAAAAA)GTACCGAGGCTTGCCAGGGGCGGCTCAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606123235_ziyi AGGGCCCACTGAGCCGCCCCTGGCAAGCCTCG(+AAAAAA)GTACCACCTGCCCAGGGCTTGCTCATCCTTG NGG 10 8 17 8 24 0 100 5 10