Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606123159_xxl0 CAGGAGGGTAGTTTTGTCCATGTGGTAGCCAG(+AAAAAA)GTACCAGAAGTCTCTGGCACAGAGCAATGTG NGG 10 8 17 8 24 0 100 5 10
    240606123158_avvd GTTTTCACATTGCTCTGTGCCAGAGACTTCTG(+AAAAAA)GTACCTGGCTACCACATGGACAAAACTACCC NGG 10 8 17 8 24 0 100 5 10
    240606123157_kozn TCTCCCCATCCTAGAAAGAGCAGAGCAATTAG(+AAAAAA)GTACCACGTTTTCTTGTTTCCCAATTCTTTA NGG 10 8 17 8 24 0 100 5 10
    240606123156_9lpp CAAGTTAAAGAATTGGGAAACAAGAAAACGTG(+AAAAAA)GTACCTAATTGCTCTGCTCTTTCTAGGATGG NGG 10 8 17 8 24 0 100 5 10
    240606123154_xcx8 TCGTTTTAGCTGATCGTCGACTGTCAGAGTAG(+AAAAAA)GTACCTCTTCTAATGTATTGCCAAAAGCATC NGG 10 8 17 8 24 0 100 5 10
    240606123153_9fpl CAACAGATGCTTTTGGCAATACATTAGAAGAG(+AAAAAA)GTACCTACTCTGACAGTCGACGATCAGCTAA NGG 10 8 17 8 24 0 100 5 10
    240606123152_0x1b CAGATAATTACTATCCCGGAAAACCTAGAGAG(+AAAAAA)GTACCCTGAGCCAGAACCCCTCAGCTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240606123150_qka5 TCTGTTGCTCAGCTGAGGGGTTCTGGCTCAGG(+AAAAAA)GTACCTCTCTAGGTTTTCCGGGATAGTAATT NGG 10 8 17 8 24 0 100 5 10
    240606123148_alqc TAGGCCTGTGCCATACCAAATATTCACCAATG(+AAAAAA)GTACCCTCTAGAGTAAAGCAAAGCTAATAAA NGG 10 8 17 8 24 0 100 5 10
    240606123147_fr8z CAACTTTTATTAGCTTTGCTTTACTCTAGAGG(+AAAAAA)GTACCATTGGTGAATATTTGGTATGGCACAG NGG 10 8 17 8 24 0 100 5 10